Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X) A total of 480 strips are provided 100 cassettes 125mL of buffer
Detail
Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X)
Blank lateral flow strips are the perfect companion during the development and feasibility testing of a lateral flow device. IN THE BEGINNING, the capture and test line reagents can be spotted onto the nitrocellulose portion of the blank strips at different concentrations, volumes and buffer conditions. This enables the user to determine the optimal conditions for subsequent spraying and enables the user to verify the test and controls will respond according to expectations before scaling up the process.
These blank strips are compatible with all of our colloidal gold products AND:
Lateral flow cassettes: C02022
Streptavidin CdSe/ZnS Quantum Dots For Lateral Flow: AU2043
Streptavidin Europium Chelate Microspheres For Lateral Flow: AU2050
Streptavidin Colloidal Gold For Lateral Flow: AU2017
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
Bind and elute all RNA irrespective of size or GC content, without bias
No phenol extractions
No Proteinase K treatment
No carrier RNA
Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purify high-quality RNA in 15-20 minutes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Exosomes purified using Norgen’s Purification Kits and most other methods
Size of RNA Purified
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
