Introduction
029110 Sterile Liquid Paraffin
Usage:For covering a liquid medium for anaerobic culture.
Specification:10ml.
10ml
029110 Sterile Liquid Paraffin
Usage:For covering a liquid medium for anaerobic culture.
Specification:10ml.
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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UltraRanger 1kb DNA Ladder (Cat# 12100) – 100 loads
Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
| Fragment | Size (bp) | Mass (ng) |
| 1 | 24000 | 30 |
| 2 | 14000 | 27 |
| 3 | 10000 | 60 |
| 4 | 8000 | 44 |
| 5 | 6000 | 36 |
| 6 | 5000 | 55 |
| 7 | 4000 | 49 |
| 8 | 3000 | 39 |
| 9 | 2500 | 35 |
| 10 | 2000 | 30 |
| 11 | 1500 | 33 |
| 12 | 1000 | 10 |
| 13 | 700 | 21 |
| 14 | 500 | 18 |
| 15 | 300 | 11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Attogene’s Carbon Dioxide Enzymatic Assay Kit is a simple, direct method for measuring Carbon Dioxide levels in the environment. The assay uses a coupled enzyme assay to detect CO2 (as HCO3-) as follows. In the first step, the bicarbonate condenses with phosphoenol pyruvate to form oxalate (and phosphoric acid); this reaction is catalyzed by the enzyme Phosphoenolpyruvate Decarboxylase, PEPC. The oxalate is then enzymatically reduced by the enzyme Malate Dehydrogenase (using an NADH cofactor) to form malate and NAD+.
Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.
Our 384-well plate has a rigid, fully-skirted frame which remains stable during thermal cycling.
The rigidity of the plate lends itself to automated and high-throughput PCR environments where robotic handling maybe in use.
The 2-component design reduces the risk of evaporation due to a poor sealing, commonly seen with 1-component PCR plates. 100% virgin medical grade polypropylene is used for the 384 wells, with a maximum capacity of 45 µL Certified free from DNase, RNase, nucleases and human gDNA.
Compatible with a wide range of PCR and qPCR applications, for a full list see ‘Plate Instrument Compatibility List’.
Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.