Listeria monocytogenes Quantified Bacterial DNA Standard
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Detail
Overview
Quantified standard to be used as a positive control or PCR quantification standard
Vigorously quantified using multiple methods
Listeria monocytogenes has emerged as a significant foodborne pathogen that poses a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium. Due to its ability to survive high and low temperatures as well as low pH, it could resist various food processing technologies, as well as grow at food storage temperatures. L. monocytogenes is known to be associated with raw meat, unpasteurized milk and dairy products, vegetables, and seafood. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals being the most susceptible.
Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Listeria monocytogenes.
Upon receipt, store Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20oC or lower.
Other Products
Plasma/Serum Exosome Purification and RNA Isolation Kits
Product Info
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Product Info
Overview
Purification and enrichment of intact plasma/serum exosomes for functional studies
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
Versatile plasma/serum input volume
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents, nor overnight incubation required
Compatible with plasma/serum from most species
Pure exosomes are purified and are free from any other RNA-binding proteins
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit
For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit
For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit
For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35 – 40 minutes
Average Yields
Variable depending on specimen
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
ProbeSure Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.
ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
