DBCO-C3-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-C3-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Purification and concentration of RNA（mRNA>200nt or miRNA）from transcription products, DNase cleavage products, labeled products, and crude RNA products
Applications	Sequencing, ligation, enzyme digestion, RT-PCR, labeling, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Crude RNA, enzymatic reaction solution
Sample amount	≤100μl
Recovery	90%
Elution volume	≥15μl
Time per run	≤15 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.

Advantages

• High recovery – recovery of RNA up to 90%
• Low elution volume – the elution volume can be as low as 15µl
• Fast – only 10 minutes for recovery by using column method
• General – suitable for various crude RNA products
• Wide range of fragment recovery:(>25nt RNA)

Kit Contents

Contents	R214402	R214403
Purification Times	50 Preps	250 Preps
Buffer GXP	30 ml	120 ml
Buffer RW2	20 ml	2 x 50 ml
RNase-Free Water	20 ml	60 ml
HiPure RNA Mini Columns I	50	250
2 ml Collection Tubes	50	250

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.