Propargyl-PEG4-methane is a crosslinking reagent that can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-methane is a crosslinking reagent that can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For the selective isolation and culture of Listeria monocytogenes.
Peptone provides the carbon and nitrogen sources necessary for growth; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride can maintain a balanced osmotic pressure; glucose provides a carbon source; Listeria hydrolyzes esculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; mannitol is a fermentable sugar, and phenol red is a pH indicator; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
| Ingredients | /liter |
| Peptone | 23 g |
| Starch | 1 g |
| NaCl | 5 g |
| Columbia agar | 13 g |
| Mannitol | 10 g |
| Ferric ammonium citrate | 0.5 g |
| Esculin (aesculin) | 0.8 g |
| Dextrose (glucose) | 0.5 g |
| Lithium chloride | 15.0 g |
| Phenol red | 0.08 g |
| pH7.2±0.2 at 25°C | |
Weigh 68.9g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and set aside. Heat to dissolve agar before use, cool to 50℃, add 1 tube of supporting reagent (SR0140) per 100mL of basal culture medium, shake well and pour into sterilized culture dish.
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Listeria monocytogenes ATCC19115 | Gray-green colonies with a black depression in the center and black surrounding |
| Enterococcus faecalis ATCC29212 | – |
| Escherichia coli ATCC25922 | – |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Peptone provides the carbon and nitrogen sources necessary for growth; yeast ex……
Product Highlights
Speed: Adjustable speed, up to 4500 rpm
Vortex Oscillation Intensity:1-4 intensity levels, selectable and adjustable
Programming:Programmable,freely choose combinations of oscillation strength,duration.centrifugation speed, and time
Safety:Safety interlock device to ensure user safety
Compatibility: Single tube or multi-tube centrifugation/mixing with one-button operation, saving time and cost
Display:Touch buttons and an LCD screen clearly indicate and set oscillation level, duration, and cycle count
Convenience: Multi-functional, compact design, occupies minimal space
Technical Specifications
| Dimensions (mm) | 200(w)x243(H)x127(D) | Working temperature | 5℃ to 40℃ |
| Oscillation Duration | 0-30 seconds (increment of 1 second) | TD4Z Speed range | 1000-3600 rpm (increment of 100 rpm) |
| Power Supply | AC 220V 50/60 Hz | Spin Timer | 0 seconds to 30 minutes |
| Weight | 3.5kg (including power supply) | Oscillation Intensity | Level 1-4 |
| Speed range TD4Z | 1-99 programs | Centrifugation Adjustment TD4Z | 1000-3600 rpm (increment of 100 rpm) |
PCR laboratories and general molecular biology laboratories
Model:
TD4Z
Brand:
Yingtai
Availability: Pre Order
Product Description
Highly Sensitive Isothermal Amplification Kit with Fast Reaction Time
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
