Introduction

MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

Details

Specifications

Features	Specifications
Main Functions	Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP)
Applications	NGS, DNA library
Purification technology	Magnetic beads technology
Process method	Manual (centrifugation or vacuum)
Sample type	DNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount	Appropriate
Recovery	80%
Operation time	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.

Advantages

• High recovery – up to 80%
• High throughput – using magnetic beads purification technology

Kit Contents

Contents	BP-5	BP-50	BP-500
MagPure A4 XP	5 ml	50 ml	500 ml

Storage and Stability

MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.

DO NOT FREEZE.

MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

DBCO-PEG6-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG6-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Extract total RNA (including microRNA) from FFPE samples
• No phenol extraction step
• Includes DNase for optional on-column DNA removal
• Isolated RNA is of the highest quality and integrity
• Isolate a diversity of RNA species
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.

FFPE RNA Purification Kit (Spin Column)

Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

FFPE RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	400 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:	5 slices of < 20 µm thick paraffin  25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Component	Cat. 25300 (50 preps)	Cat. 25400 (2 x 96 preps)
Digestion Buffer A	25 mL	2 x 25 mL
Buffer RL	30 mL	2 x 30 mL
Enzyme Incubation Buffer	6 mL	2 x 6 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 20 mL
Proteinase K	12 mg	2 x 20 mg
DNase I	1 vial	2 x 500μL
Micro Spin Columns	50	–
96-Well Incubation Plate	–	2
96-Well Plate	–	2
Adhesive Tape	–	8
Collection Tubes	50	–
96-Well Collection Plate	–	2
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1