Name of Product Megasphaera/Pectinatus Screen Catalog Number MGScMP 1 Short Info GenLine discovers beer germs in a timely and precise manner
Method/Platform PCR Range/Assay Sensivity 10^4 – 10^5 KBE/mL Test Principle The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Detail
Name of Product
Megasphaera/Pectinatus Screen
Catalog Number
MGScMP 1
Short Info
GenLine discovers beer germs in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 KBE/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Brief Instructions
The entire procedure of the test can be divided into 3 different steps.
1.The PCR reagents and the samples are prepared.
2.After the addition of the sample to the PCR reagents, the PCR is started.
3.The resulting PCR products are detected by a simple lateral flow test Strip
EXTRAClean Urine Exosome Purification and RNA Isolation Kits
Product Info
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Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact urinary exosomes for functional studies.
Bind and elute all RNA irrespective of size or GC content, without bias.
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
No phenol extractions, Proteinase K treatment, nor carrier RNA required.
No time-consuming ultracentrifugation, filtration nor special syringes are required.
No precipitation reagents nor overnight incubation required.
Compatible with urine from any species.
Pure exosomes are purified and are free-from any other RNA-binding proteins.
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.
Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.
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endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.
Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
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Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
