Attogene’s Mercury Lateral Flow Kit can be used for the screening of Mercury in water and food (fish) samples at 10 ppb in a laboratory setting. Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting. The instructions include method for extraction from food samples.
Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs and immune system problems for adults, and the physical and mental development delays in infants and children. Attogene’s Mercury Lateral Flow test gives results conforming of 10ppb or greater. Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount
1-1.5ml whole blood, 0.5-1ml bone marrow
Yield
1-30μg
Principle
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.
Advantages
High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
Economy – less than 50% of the price of top brands
Automatic – extraction without manual participation, saving time and effort
High yield – nucleic acid recovery up to 95%
General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.
Kit Contents
Contents
R661101
R661102
R661103
Purification Times
48 Preps
96 Preps
480 preps
10 x RBC Lysis Buffer
50 ml
2 x 50 ml
4 x 100 ml
Proteinase K
12 mg
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
1.8 ml
10 ml
DNase I
600 μl
2 x 600 μl
10 x 600 µl
DNase Buffer
20 ml
30 ml
150 ml
MagPure Particles N
1.2 ml
2.5 ml
11 ml
Buffer RTL
30 ml
60 ml
300 ml
Buffer ALB2
40 ml
60 ml
300 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.
Document
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
