Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
Can process challenging samples such as mastitic milk
Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
Fast and efficient spin-column format
This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk. Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.
*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.
Component
Cat. 21550 (50 preps)
Resuspension Solution A
6 mL
Buffer SK
60 mL
Wash Solution A
18 mL
Elution Buffer B
15 mL
Proteinase K
12 mg
Lysozyme (powder)
120 mg
Spin Columns
50
Collection Tubes
50
Elution Tubes (1.7 mL)
50
Product Insert
1
Other Products
P1156 HiPure Plasmid EF Maxi Kit
Product Info
Document
Product Info
Introduction
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications
Cell transfection, animal injection, etc.
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid vector
Sample amount
High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium
Yield
200-1500μg
Elution volume
≥0.7ml
Time per run
≤60 minutes
Liquid carrying volume per column
20ml
Binding yield of column
1mg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 1.5mg plasmid can be binded in one column
Ultra low endotoxin – content of endotoxin is less than 0.1 EU/μg, which can be directly used for cell transfection and animal injection, etc.
Kit Contents
Contents
P115602
P115603
Purification Times
10 Preps
50 Preps
RNase A
30 mg
150 mg
Buffer P1
100 ml
500 ml
Buffer P2
100 ml
500 ml
Buffer LN3
50 ml
250 ml
Buffer PW1
33 ml
180 ml
Buffer PW2
20 ml
100 ml
Elution Buffer
15 ml
120 ml
Buffer CL
33 ml
180 ml
HiPure DNA Maxi Columns C
10
50
Lysate Clear Midi Syringe
10
50
50 ml Collection Tubes C
20
100
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Staphylococcus Aureus PCR Pathogen Detection Kit For Isothermal Fluorescence Detector
Product Info
Document
Product Info
Product Description
Product Description:
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Amp-future bio, a leading Chinese manufacturer of detection kits, is proud to introduce its new Pathogen Detection Kit. This innovative swab test kit is specially designed for rapid detection of influenza and other infectious diseases, delivering results in as little as 5-20 minutes. Featuring a storage temperature of -20ºC, this test kit is highly reliable and easy to use. It is ideal for hospitals, medical centers, and other healthcare facilities to quickly and accurately detect pathogens. As a trusted name in the industry, Amp-future bio guarantees the highest quality and safety when it comes to its Pathogen Detection Kit, making it the perfect choice for all your rapid test kits and infectious disease test kits needs.
Support and Services:
Pathogen Detection Kit technical support and services are offered to customers to ensure optimal performance and usability of the product. Technical support is available through our customer service hotline as well as our online customer portal. Our technical support team is available to answer any questions and provide guidance for product installation, troubleshooting, and maintenance. Additionally, our team provides software updates and upgrades, as well as access to our library of technical documents and resources. We also offer training and education services for customers who wish to better understand the product and its capabilities.
Packing and Shipping:
To ensure that our Pathogen Detection Kit arrives to you in perfect condition, we take special care in packaging and shipping.
Before shipping, all kits are carefully inspected for product quality. They are then bubble-wrapped and sealed in a box to provide the highest level of protection.
We use a reputable shipping company with tracking options and signature confirmation. Depending on the shipping destination, the package may require additional documentation, such as a Certificate of Origin or an invoice.
We strive to deliver your order as quickly and cost-effectively as possible. We offer same day shipping for orders placed before noon EST.
FAQ:Q1: What is Amp-future bio Pathogen Detection Kit?A1: Amp-future bio Pathogen Detection Kit is a high-performance product developed by China for rapid detection of pathogenic microorganisms.Q2: What microorganisms can be detected by this kit?A2: The Amp-future bio Pathogen Detection Kit can detect a wide range of pathogenic microorganisms, including bacteria, viruses, and parasites.Q3:Is the kit easy to use?A3: Yes. The Amp-future bio Pathogen Detection Kit is designed for easy operation and use. It comes with detailed instructions for use.Q4: Is the kit safe?A4: All components of the Amp-future bio Pathogen Detection Kit are strictly tested to ensure safety. The kit is safe and reliable to use.
Document
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
