The micro porous plate centrifuge adopts international advanced design concepts and manufacturing technology, with a smooth and beautiful appearance, compact and stable structure, adhering to the advantages of high universality and easy operation. It also has the functional characteristics of “smooth start” and “smooth braking”; This micro porous plate centrifuge is very suitable for 96 or 384 well and small capacity micro porous plates, as well as various standard PCR micro porous plates with or without skirts .
Detail
Product advantages
1. The instantaneous centrifugal function rises to the maximum speed for about 6 seconds, allowing for rapid detachment of hanging droplets from the wall;
2. Faster acceleration and deceleration rate, required to complete the experiment in a shorter time;
3. Door cover protection, overspeed and imbalance detection system, capable of real-time monitoring of the centrifugal process, ensuring the safe operation of the instrument; When the operation ends, an error occurs, or an imbalance occurs, the sound signal prompts, and the operation stops at the same time. The LCD displays the result code
Host Parameters
Product model
Mini-3
Input power supply
DC24V/2.75A
Input power
55W
Motor/Drive Method
DC24V brushless DC variable frequency motor
Display method
High brightness LCD screen
Effective centrifugal time
1-99min.1-59sec
speed
300~3000±15rpm
Speed step increase
10rpm
Maximum capacity
2 *96 well PCR plates/ELISA plates
MAX. RCF
608 xg
Misoperation/alarm failure
Sound prompt+display code
weight
3.9Kg
Noise
60dB (A)
Fastest acceleration time
<6s
Fastest deceleration time
<5s
Permissible ambient temperature/relative temperature
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
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Exceptional value for money Rapid detection of 2019-nCoV Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate
Applications
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method
96 well Bind Plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
PCR product, enzymatic reaction
Sample amount
Appropriate
Recovery
≥80%
Elution volume
≥75μl
Time per run
≤60 minutes
Liquid carrying volume per column
800µl
Binding yield of column
20µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – ≥80% DNA recovery
Fast – isolation can be completed in 60 minutes
Kit Contents
Contents
D212201
D212202
D212203
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
Buffer GDP
120 ml
400 ml
4 x 400 ml
Buffer DW2
20 ml
100 ml
3 x 100 ml
Elution Buffer
20 ml
20 ml
30 ml
HiPure DNA Plate
1
4
20
2.2ml Collection Plate
1
4
20
1.6 ml Collection Plate
1
4
20
0.8 ml Collection Plate
1
4
20
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Document
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
