Overview
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments
- Double intensity reference bands at 200 bp and 400 bp
The Norgen MiniSizer 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments with double intensity reference bands at 200 bp and 400 bp. The MiniSizer is ideal for PCR product size confirmation.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
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MiniSizer 50 bp DNA Ladder (Cat# 11200) – 100 loads
Ladder Properties:
• Fourteen discrete bands, ranging from 50 bp to 650 bp
• Higher intensity bands at 200 bp and 400 bp for easy reference
| Fragment | Size (bp) | Mass (ng) |
| 1 | 650 | 61 |
| 2 | 600 | 46 |
| 3 | 550 | 36 |
| 4 | 500 | 30 |
| 5 | 450 | 35 |
| 6 | 400 | 85 |
| 7 | 350 | 27 |
| 8 | 300 | 25 |
| 9 | 250 | 16 |
| 10 | 200 | 54 |
| 11 | 150 | 33 |
| 12 | 100 | 21 |
| 13 | 50 | 17 |
| 14 | 25 | 14 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
