Multiplexing Index Primers (Illumina Platform)

Overview

• Detection kits for Cryptosporidium
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.

Cryptosporidium TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Cryptosporidium TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

Figure 1 / 3

Previous

Next

Click for expanded view

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM39150 (100 preps)	Cat. TM39110 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Cryptosporidium Primer & Probe Mix	280 μL	280 μL
Cryptosporidium Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Introduction

This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from tissue, cell, whole blood
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	Tissues, cells, lymphocytes and other clinical sample
Sample amount	Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture

Kit Contents

Contents	IVD3020B-96	IVD3020B
Purification Times	96 Preps	200 Preps
MagPure Particles N	2.5 ml	5 ml
DNase I	2 x 600 µl	4 x 600 µl
DNase Buffer C	60 ml	120 ml
Buffer RLC	60 ml	120 ml
Buffer MCB*	60 ml	150 ml
Buffer GW1*	44 ml	110 ml
Buffer MW2*	50 ml	100 ml
RNase Free Water	20 ml	60 ml

Storage and Stability

DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.

This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.

Introduction

Usages:

For cultivations of yeasts , moulds and acidophilic bacteria.

Principle:

Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.

Formulation(per liter):

Peptone 5g

Tryptone 5g

Glucose 20g

Final pH 5.7 ± 0.1        

How to use:

1.Suspend 30g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.

2.Diluted and treated samples.

Quality control:

Item	The name and number of strain	Growth	Colony Color
1	Aspergillus niger ATCC16404	Good	Spore surface growth
2	Candida albicans ATCC10231	Good	Cloudy broth
3	Escherichia coli ATCC25922	Inhibition	—

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g