N-(Acid-PEG2)-N-bis(PEG2-propargyl) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Acid-PEG2)-N-bis(PEG2-propargyl) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Usages:
For cultivating of bacteria.And sterility test of drugs and biological products.
Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.
Formulation（per liter）:
Pancreatic Digest of Casein 17g
Papaic Digest of Soybean 3g
Sodium chloride 5g
Dipotassium hydrogen phosphate 2.5g
Glucose Monohydrate 2.5g
Final pH 7.3±0.2
How to use：
1.Suspend 30g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g
These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.
Stool DNA Isolation Kit (Spin Column)
Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation 96-Well Kit (High Throughput)
Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation Kit (Magnetic Bead System)
Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).
Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.
Free Download
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
Figure 1 / 12
Click for expanded view
| Kit Specifications | |
| Maximum Stool Input | 200 mg (fresh/frozen stool) or 400 μL (preserved stool) |
| Type of Stool Processed | Frozen, fresh or preserved stool |
| Format | Spin Column |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Elution Volume | 50 μL |
| Time to Complete 10 Purifications | 30 minutes |
| Applications | PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis. |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 27600 (50 preps) | Cat. 65600 (192 preps) | Cat. 55700 (50 preps) | Cat. 63100 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer L | 60 mL | 2 x 105 mL | 60 mL | 3 x 60 mL 1 x 30 mL |
| Lysis Additive A | 6 mL | 25 mL | 6 mL | 25 mL |
| Binding Buffer I | 7 mL | 25 mL | 7 mL | 25 mL |
| Binding Buffer C | 30 mL | 110 mL | – | – |
| Wash Solution A | 18 mL | 2 x 38 mL | – | – |
| Magnetic Bead Suspension | – | – | 1.1 mL | 4 x 1.1 mL |
| Solution WN | – | – | 18 mL | 55 mL |
| Elution Buffer B | 8 mL | 30 mL | 8 mL | 30 mL |
| Bead Tubes | 50 | 200 | 50 | 196 |
| Mini Spin Columns | 50 | – | – | – |
| 96-Well Plate | – | 2 | – | 2 |
| Adhesive Tape | – | 4 | – | 2 |
| Collection Tubes | 50 | – | – | – |
| 96-Well Collection Plate | – | 2 | – | – |
| Elution Tubes (1.7 mL) | 50 | – | 50 | – |
| 96-Well Elution Plate | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
TDL5E Bench top low speed refrigerated centrifuge machine
Application:
Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
TDL5E Technical Parameters:
| Max. Speed | 5000rpm | Max.RCF | 4420xg |
| Max. Volume | 4x180ml | Speed Accuracy | ±20rpm |
| Timer | 1~99h59min | Noise | ≤60dBA |
| Dimension(LxWxHmm) | 570x600x340mm | Net Weight | 76Kg |
| Power Supply | AC220V/110V 50HZ/60HZ | Temperature Range | -20℃~40℃ |
| Temperature Accuracy | ±1℃ | Certifications | CE, ISO & Calibration report is available. |
Matched Rotors for TDL5E:
| Order No. | Rotor Type | Max. Speed(rpm) | Volume(ml) | Max.RCF(xg) |
| 5E-1 | Swing rotor | 4000 | 4x180ml | 2580 |
| 5E-2 | Microplate rotor | 4000 | 2x3x96well | 2020 |
| 5E-3 | Swing rotor | 5000 | 4x1x50ml | 4420 |
| 5E-4 | Swing rotor | 5000 | 4x1x100ml | 4420 |
| 5E-5 | Swing rotor | 4000 | 4x2x50ml | 2820 |
| 5E-6 | Swing rotor | 4000 | 4x4x15ml | 2820 |
| 5E-7 | Swing rotor | 4000 | 4x6x10/7ml | 2580 |
| 5E-8 | Swing rotor | 4000 | 4x6x5ml | 2170 |
| 5E-9 | Swing rotor | 4000 | 4x4x10/7ml | 2580 |
| 5E-10 | Swing rotor | 4000 | 4x4x5ml | 2170 |
| 5E-11 | Fixed rotor | 5000 | 6x15ml | 2540 |
| 5E-12 | Fixed rotor | 5000 | 12x15ml | 3080 |
| 5E-13 | Fixed rotor | 5000 | 24x15ml | 3500 |
| 5E-14 | Fixed rotor | 5000 | 4x50ml | 2520 |
| 5E-15 | Fixed rotor | 5000 | 6x50ml | 2850 |
| 5E-16 | Fixed rotor | 5000 | 4x100ml | 2630 |
| 5E-17 | Fixed rotor | 5000 | 6x100ml | 3130 |
TDL5E is a table top low speed refrigerated centrifuge widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
