N-bis(PEG2-propargyl)-N-(PEG2-amidoPEG1)-N-(bis(PEG2-propargyl)

Overview

Virgin Polypropylene 96 Square Well plate with high uniformity from well to well.

• Working Volume of 2.0ml
• Square well with ‘V’ bottom for maximum liquid extraction
• High uniformity from well to well
• Raised well rims for reliable closing with heat sealing.
• Easy and reliable stacking
• Good centrifugation stability up to 6,000 × g for faster protocols and improved sample quality

Virgin Polypropylene 96 Square Well plate with high uniformity from well to well.

Overview

• Fast and easy high throughput processing using either a vacuum manifold or centrifugation
• Process all soil types including clay, loam, sandy soils and high humic content soils such as peat, compost and manure
• Remove organic substances using the OSR Solution
• Remove all humic acid from DNA samples using the Humic Acid Removal plate
• Isolate high quality total DNA from all soil types – ready for any downstream PCR, qPCR
• ​Excellent DNA for metagenomic studies
• No phenol or chloroform extractions

This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR  applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.

Details

Supporting Data

Figure 1 / 2

Previous

Next

Click for expanded view

Kit Specifications
Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of DNA Purified	All sizes
Maximum Amount of Starting Material	250 mg
Time to Complete 96 Purifications	50 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 26560 (192 preps)
Lysis Buffer G	2 x 100 mL
Lysis Additive A	25 mL
Binding Buffer I	25 mL
OSR Solution	12 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
Bead Tubes	200
HAR Plate	2
96-Well Filter Plate	2
Adhesive Tape	4
96-Well Collection Plate	4
96-Well Elution Plate	2
Product Insert	1