Introduction

This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.

Details

Specifications

Features	Specifications
Main Functions	Extract total pathogen nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant.
Applications	RT-PCR，PCR
Products	Pathogen DNA / RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant
Sample amount	200-300μl
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.

Advantages

• Fast – several samples can be extracted in 40 minutes by column method
• High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

Kit Contents

Contents	IVD6672-50	IVD6672
Purification Times	50 Preps	200 Preps
Bead Tube C	50	4 x 50
MagPure Particle	1.6 ml	7.0 ml
Proteinase K	24 mg	100 mg
Protease Dissolve Buffer	1.8 ml	6 ml
Buffer MLBN	30 ml	120 ml
Buffer MW1*	22 ml	53 ml
Buffer MW2*	20 ml	50 ml
Buffer NFW	10 ml	30 ml

Storage and Stability

MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.

Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

K-GLUT

SKU: 700004301

60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser)

Content:	60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	L-Glutamic Acid, MSG
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	492
Signal Response:	Increase
Linear Range:	0.4 to 20 µg of L-glutamic acid per assay
Limit of Detection:	0.21 mg/L
Reaction Time (min):	~ 8 min
Application examples:	Fruit and vegetables (e.g. tomato), processed fruit and vegetables (e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by ISO, GOST and NMKL

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

Explore more organic acid test kits.

Advantages

• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Glutamate dehydrogenase solution stable below -10^oC  
• No wasted diaphorase solution (stable suspension supplied)  
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.