N-Boc-N-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are combined together at a Boc-protected secondary amine. Terminal alkynes are most commonly used in copper click chemistry with azides to form stable triazoles with the target molecule. The secondary amine may be deprotected under acidic conditions to allow for alkylation at that position, increasing the molecule’s structural complexity. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.
Detail
N-Boc-N-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are combined together at a Boc-protected secondary amine. Terminal alkynes are most commonly used in copper click chemistry with azides to form stable triazoles with the target molecule. The secondary amine may be deprotected under acidic conditions to allow for alkylation at that position, increasing the molecule’s structural complexity. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.
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Saliva DNA Collection and Preservation Devices (50)
Product Info
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Product Info
Overview
Reliable and cost-effective
Non-invasive, user-friendly sample collection and preservation in one convenient kit
Preserved DNA is stable for 2 years at ambient temperature
High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
Norgen’s Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components:
Saliva Collection Funnel and Collection Tube
Collection Tube Cap
Norgen’s Saliva DNA Preservative contained within a sealed squeezable ampoule
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.
Saliva DNA Preservative
Norgen’s Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.
When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.
Kit Components
Cat. RU49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices
50
Donor Procedure Flowchart
1
Product Insert
1
Individual Saliva DNA Collection and Preservation Device Contents
Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
Isolate total RNA (including microRNA) without phenol
Isolated RNA is of high quality, integrity and diversity
Also available in 96-well format for high throughput applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
* Yield will vary depending on the type of sample processed.
Kit Specifications – 96-well
Maximum Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:FungiPlant Tissues
40 mg40 mg
Average Yield* 40 mg of Grape Leaves 40 mg Tomato Leaves 40 mg Tobacco Leaves 40 mg Peach Leaves
5-7 μg 20-30 μg 20-30 μg 15-20 μg
Time to Complete 96 Purifications
30 minutes
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
SMOBIO’s B-BOX™ is a long wavelength, blue light LED epi-illuminator. It is compact in design and robust in structure. The B-BOX™ epi-illuminator provides an unprecedented level of safety for its user due to its non-UV light source and a low operating voltage of only 12 Volts, as well as its capability working with non-carcinogenic DNA/ protein dye. The B-BOX™ comes with high quality non-flickering LED light that is highly sensitive for DNA and protein dye. B-BOX™ can greatly ease the routine chore of gel extraction, enabling easy visualization and gel cutting even in bright ambient light. The multi-angle filter plate (Cat. No. VE0102) provides optimal angles for gel cutting, visualization, and documentation. The B-BOX™ also comes with amber colored filter goggles (Cat. No. VE0103) for gel cutting and visualization. The Phox™ Photobox (Cat. No. VE0104) provides a mini darkroom environment for images taken with any smartphone. Finally, a built-in barrier system around the working area helps facilitate cleaning.
Features
Improved cloning efficiency
Compact, lightweight, and portable (less than 1 kg in weight)
Safety features include : 470 nm long wavelength, without any UV radiation hazard to its user
Compatible with non-carcinogenic, non-ethidium bromide DNA staining dye
User friendly: Samples are easy to visualize (when using the filter plate or goggles)
LED light source lasts up to 50,000 hours
Superior detection sensitivity: ≤ 0.04 ng of DNA when using FluoroStain™ DNA Fluorescent Staining Dye, ≤3 ng of protein when using FluoroStain™ Protein Fluorescent Staining Dye (as sensitive as silver stain)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation
Built-in barrier design, for easy clean up
Visible in bright ambient light
Emphasizes minimal power reliance, low heat generation, with its own built-in heat sink
Document
SMOBIO’s B-BOX™ is a long wavelength, blue light LED epi-illuminator. It is compact in design and robust in structure. The B-BOX™ epi-illuminator provides an unprecedented level of safety for its user due to its non-UV light source and a low operating voltage of only 12 Volts, as well as its capability working with non-carcinogenic DNA/ protein dye. The B-BOX™ comes with high quality non-flickering LED light that is highly sensitive for DNA and protein dye. B-BOX™ can greatly ease the routine chore of gel extraction, enabling easy visualization and gel cutting even in bright ambient light. The multi-angle filter plate (Cat. No. VE0102) provides optimal angles for gel cutting, visualization, and documentation. The B-BOX™ also comes with amber colored filter goggles (Cat. No. VE0103) for gel cutting and visualization. The Phox™ Photobox (Cat. No. VE0104) provides a mini darkroom environment for images taken with any smartphone. Finally, a built-in barrier system around the working area helps facilitate cleaning.
