N-(Boc-PEG1)-N-bis(PEG2-propargyl)

Introduction

Usages:

For the rapid detection and of coliforms and E. coli.

Principle:

Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate were mixed occurrence of coliforms and E. coli enzyme corresponding specific reactions, hydrolysis of the substrate, the release of the color groups, in a pale yellow plates coliforms appears orange-red colonies while E.coli appears blue-green colonies.

Formulation (per liter):

Peptone 15.0g

Yeast extract powder 3.0g

Sodium chloride: 5.0g

Sodium lauryl sulfate: 0.1g

Agar: 12.0g

Mixed chromogenic substrate: 6.77g

Final pH 7.0 ± 0.2

How to use:
1. Weigh 41.9g of the product, adding , 1.0 L of distilled or deionized water, heated to boiling stir until completely dissolved, dispensing into flask, 115 autoclaved 10minutes.

2, Take 25.0g or 25.0mL of sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate buffered saline (or saline) ,shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 medium.

3, observe the results.

Quality control:

This product appears light yellow after  pouring on plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.

Bacteria name            bacteria NO.       growth situation         feature

Escherichia coli          ATCC25922            good         blue-green colonies

Citrobacter              ATCC8090             good         orange-red colonies

Salmonella typhimurium   CMCC50115          good           colorless colonies

Enterococcus faecalis     ATCC29212           suppressed            —–

Storage: Store in a dark, cool and dry place, tighten the caps immediately after use. Storage period of two years.

1000mL

Features

1.        Brushless frequency motor, in great torque, free maintenance, no power pollution, quick in speed up and down.

2.        7” LCD Touch screen display which indicates the program, rotor No, speed, time, RCF and temperature.

3.        CFC-free refrigerant, pre-cooling function ,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.

4.        Microprocessor control , 100 programs in store, 10 kinds of acceleration and deceleration for your choice.

5.         Over speed and imbalance protection.

6.        Speed, RCF, time, temperature can be edited during running.

7.        High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve, 3 tiers protection, safe and reliable.

8.        Automatic door open & close, as well as anti-pinch function (Optional).

CDL7M Technical Parameter：

Max. Speed	7000rpm
Max. RCF	11650×g
Max. Capacity	6×2400ml (12*500ml blood   bag）
Time Range	1~99h59min59s
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Temperature	-20 ~ 40℃
Acc/Dec	10 Kinds
Speed   Accuracy	±20r/min
Temperature   Accuracy	±1℃
Voltage(V/Hz)	AC 220V/380V    50HZ/60HZ
Size (W x D x Hmm)	940×890×1000mm
Net Weight(Kg)	570KG
Certificates	CE,ISO & Calibration report are available

Special rotor for blood bag separation

Order   No.	Rotor   No.	Max   Speed (rpm)	Max Volume(ml)	Max.   RCF（×g）
05202	Swing Rotor	4000	6×2x1000ml	5210

CDL7M touch screen with special programs for blood bag separation, can get good performance for platelet, red cell, all parts separation of blood. 6x2x1000ml bucket suitable for blood bag separation. Also with automatical lid lock open and close function, also wind shield can open along with door open.
Model:
CDL7M

Brand:
Yingtai

Code:
CDL7M blood bank centrifug

Overview

• Detection kits for Staphylococcus aureus
• Available in TaqMan format for analysis

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry (Lee et al., 2005). S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulasepositive, which forms the basis of traditional identification methodology.

Staphylococcus aureus TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Staphylococcus aureus TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM29350 (100 preps)	Cat. TM29310 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
S. aureus Primer & Probe Mix	280 μL	280 μL
S. aureus Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1