Description

Fentanyl (FYL) Detection Kit (Rapid Lab) is a lateral flow chromatographic immunoassay for the qualitative detection of Fentanyl/Norfentanyl in liquid and powder substances at the cut-off concentration of 200 ng/mL.

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It is specific for Fentanyl screening with no significant cross reactivity to other opiates, such as Morphine and Heroin.

Interpretation of test results are: positive (one line), negative (two lines), invalid (no lines or no control line).

• • Sample: Liquid / Powder
• • Format: Cassette
• • Sensitivity: 200 ng/mL
• • Specificity: Fentanyl
• • Accuracy: > 98%
• • Time-to-Result: 10 minutes
• • Storage Condition: 2-30°C/36-86°F
• • Test Principle: Lateral Flow Immunoassay

For professional institutions use only, not for self-testing.

Learn More:

• Fentanyl Facts  from CDC
• Fentanyl Test Strips: A Harm Reduction Strategy

Rapid screening test for the qualitative detection of Fentanyl (FYL)
Format: 10 tests
Run Time: 10 Minutes

Introduction

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 50mg simple plant using 96 well bind plate
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤50mgDried plant / seed samples: ≤15mg
Elution volume	≥75μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
• High throughput – 96 samples can be processed simultaneously by 96 well plate
• High permeability – using composite membrane technology, no hole plugging

Kit Contents

Contents	D316702	D316703
Purification Times	4 x 96 Preps	20 x 96 Preps
RNase Solution	5 ml	22 ml
Elution Buffer	120 ml	500 ml
Buffer SPL	200 ml	2 x 500 ml
Buffer PS	100 ml	400 ml
Buffer GW1	220 ml	5 x 220 ml
Buffer GW2	100 ml	3 x 100 ml
1.6ml Collection Plate	4	20
HiPure gDNA Plate	4	20
2.2ml Collection Plate(DW Plate)	8	40
0.8ml Collection Plate(DW Plate)	4	20
Sealing Film	20	100

Storage and Stability

RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.