N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.
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N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.
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DBCO-PEG4-Gly-Gly-Gly
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Product Info
Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
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Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Usages: Determination of coliform and fecal coliform for multiple tube fermentation.
Principle: Tryptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride osmotic pressure balance can be maintained; Lactose is a coliform fermentable sugars; potassium dihydrogen phosphate and dipotassium phosphate is a buffer; lauryl sodium can inhibit the growth of non-coliform bacteria.
How to use: 1. Suspend 35.6g of the product, adding 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, packed in a test tube with a small down tube, 121 ℃ autoclave 15min, leave to cool to room temperature, standby . 2.Sample handling and dilution. 3.Selected three consecutive dilution, each dilution was inoculated three LST broth tubes, each tube was inoculated 1mL. 4. Put the tubes in an incubator 36 ± 1 ℃ cultured for 48 ± 2h. 5. Observe the results. if all LST broth don’t pruduse gas, can be reported as negative for E. coli, if gas production then will have to make further confirmed by experiments.
Quality control: Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows: Bacterial Name Bacterial No. Growth Status Gassing Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good —
Staphylococcus aureus ATCC6538 inhibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Propargyl-PEG4-bromide is a crosslinker that can be used in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage with azides. The bromide (Br) acts as a good leaving group for nucleophilic substitution reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG4-bromide is a crosslinker that can be used in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage with azides. The bromide (Br) acts as a good leaving group for nucleophilic substitution reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.