N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.
N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.
Usages：
Determination of coliform and fecal coliform for multiple tube fermentation.
Principle:
Tryptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride osmotic pressure balance can be maintained; Lactose is a coliform fermentable sugars; potassium dihydrogen phosphate and dipotassium phosphate is a buffer; lauryl sodium can inhibit the growth of non-coliform bacteria.
Formulation (per liter):
Trypticase:20g
Sodium chloride: 5g
Lactose:5g
Potassium dihydrogen phosphate: 2.75g
Dipotassium hydrogen phosphate :2.75g
Sodium lauryl sulfate 0.1g
Final pH6.8 ± 0.2
How to use:
1. Suspend 35.6g of the product, adding 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, packed in a test tube with a small down tube, 121 ℃ autoclave 15min, leave to cool to room temperature, standby .
2.Sample handling and dilution.
3.Selected three consecutive dilution, each dilution was inoculated three LST broth tubes, each tube was inoculated 1mL.
4. Put the tubes in an incubator 36 ± 1 ℃ cultured for 48 ± 2h.
5. Observe the results. if all LST broth don’t pruduse gas, can be reported as negative for E. coli, if gas production then will have to make further confirmed by experiments.
Quality control:
Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows:
Bacterial Name Bacterial No. Growth Status Gassing
Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good —
Staphylococcus aureus ATCC6538 inhibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 250g/bottle
250g
Norgen’s EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.
| Kit Specifications (Spin Column) | |
| Maximum Column Binding Capacity | 10 μg |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Maximum Amount of Starting Material | 10 μg of RNA |
| Minimum Elution Volume | 8 μL |
| Time to Complete 10 Purifications | 20 minutes |
| Average Yields | ≥ 90% |
Description
The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 25 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Features
Applications
Storage
-20°C for 36 months
The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 25 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
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