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N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-methyl ester)

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N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Detail

N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Other Products

Hepatitis C Virus (HCV) TaqMan RT-PCR Detection Kits

Overview

  • Detection kits for HCV
  • Available in TaqMan format for analysis

Hepatitis C virus infection is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC) worldwide. Neither a vaccine nor an effective treatment is available for HCV. Pegylated interferon-α (PEG-IFN- α), combined with ribavirin, is the current standard therapy. Its efficiency ranges between 20-80%, depending on the HCV genotype. Unfortunately, many HCV infected patients do not respond to or do not tolerate the IFN-based therapy. Therefore, the number of patients progressing to HCC, as a result of HCV infection, is expected to increase over the next 20-30 years. The natural history of the disease reveals the elusive nature of the virus in a number of features. First, the infection is often detected incidentally at the time of blood donation as the acute infection is clinically asymptomatic in most patients. Second, HCV successfully escapes multi-specific host immune responses in the majority of patients which establishes persistent infection. Third, a significant number of persistently infected individuals remain unaware of the infection for decades, until liver fibrosis, cirrhosis and/or hepatocellular carcinoma develop.

HCV TaqMan RT-PCR Kit, 100 reactions

  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents

HCV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Figure 1. HCV TaqMan PCR Kit
Figure 1. HCV TaqMan PCR Kit
Figure 2. HCV TaqMan PCR Kit
Figure 3. HCV TaqMan PCR Kit

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

ComponentCat. TM37650 (100 preps)Cat. TM37610 (100 preps)
MDx TaqMan 2X PCR Master Mix2 x 700 μL
HCV Primer & Probe Mix280 μL280 μL
HCV Positive Control150 μL150 μL
Nuclease-Free Water (Negative Control)1.25 mL1.25 mL
Product Insert11

RevTaq RT-PCR DNA polymerase

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.

RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.

– half-life at 95°C of >40 min.

– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (<57°C)

– facilitates “zero-step” RT-PCRs directly from RNA templates (without an isothermal reverse transcription step), as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step.

– allows reverse transcription reactions at high temperatures, thus minimizing the problems encountered with strong secondary structures in RNA that melt at elevated temperatures.

– RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes

Please note: Due to the thermostable nature of the enzyme, it is recommended to design very high melting primers and probes (>65°C). See more details in FAQ.

For research use and further manufacturing.

In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.

D-Fructose/D-Glucose Assay Kit

K-FRUGL

SKU: 700004287

110 assays (manual) / 1100 assays (microplate) / 1100 assays (auto-analyser)

Content:110 assays (manual) / 1100 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 2 years under recommended storage conditions
Analyte:D-Fructose, D-Glucose
Assay Format:Spectrophotometer, Microplate, Auto-analyser
Detection Method:Absorbance
Wavelength (nm):340
Signal Response:Increase
Linear Range:4 to 80 µg of D-glucose, D-fructose or sucrose per assay
Limit of Detection:0.66 mg/L
Reaction Time (min):~ 13 min
Application examples:Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.).
Method recognition:Methods based on this principle have been accepted by AOAC Method 985.09, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

See more of our monosaccharide assay kits.

Scheme-K-FRUGL FRUGL Megazyme

Advantages

  • Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
  • PVP incorporated to prevent tannin inhibition 
  • Validated by the University of Wine, Suze la Rousse, France 
  • Very competitive price (cost per test) 
  • All reagents stable for > 2 years after preparation (manual analysis applications) 
  • Rapid reaction at either 25 or 37o
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats