NEST 2.0 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
NEST 2.0 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
Perfect for:
• End-point gel electrophoresis DNA detection
• down-stream applications (e.g. sub-cloning)
• facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQBAS01
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture. Recommend for High copy vector, Low elution volume, High concentration |
| Applications | Cell transfection, animal injection, etc. |
| Purification method | Midi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | High copy plasmid vector |
| Sample amount | 25-50ml LB |
| Yield | 10-500μg |
| Elution volume | ≥0.1ml |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 250μg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
| Contents | P123102 | P123102B |
| Purification Times | 50 Preps | 50 Preps |
| RNase A | 30 mg | 30 mg |
| Buffer E1 | 150 ml | 150 ml |
| Buffer E2 | 150 ml | 150 ml |
| Buffer E3 | 150 ml | 150 ml |
| Buffer E4 | 150 ml | 150 ml |
| Buffer E5 | 150 ml | 150 ml |
| Buffer PW2* | 50 ml | 50 ml |
| Elution Buffer | 30 ml | 30 ml |
| MaxPure EF Mini Column | 50 | 50 |
| 2 ml Collection Tubes | 50 | 50 |
| Lysate Clear Midi Syringe | 50 | 50 |
| Extend Tubes | 50 | 50 |
| 50ml Centrifuge Tubes | 50 | |
| Support Tubes | 50 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2–8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
Qubit tubes from the same lot should be used for the experiments for optimal result. 500 tubes per package.
Features
Specifications
| Shipping condition | Room temperature |
| Type | Assay tube (for Qubit fluorometer) |
| For use with (equipment) | Qubit fluorometer |
| Storage | Store at room temperature |
| Package | 500 tubes |
Qubit is a registered trademark of ThermoFisher Scientific.
The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
