NEST 2.0 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Detail
NEST 2.0 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Other Products
Large Capacity High Speed Refrigerated Centrifuge
Product Info
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Product Info
GL12 Technical Parameter:
Max. Speed
12000rpm
Max. RCF
22650×g
Max. Capacity
4×1000ml
Timer
1~9h59min
RPM/RCF Convert
Yes
Noise (dB)
≤ 60
Temperature Range
-20~40℃
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (L x W x Hmm)
780×675×830mm
Net Weight(Kg)
260KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for GL12
Order No.
Rotor No.
Max Speed (rpm)
Max Volume(ml)
Max.RCF(×g)
L12-1
Angle Rotor
12000
6×250/300ml
22650
L12-2
Angle Rotor
8000
6×500ml
11740
L12-3
Swing Rotor
4000
4×1000ml
4060
L12-4
Microplate Rotor
4000
4×4×96well
2940
L12-5
4000
2×4×96well
2490
L12-6
Angle Rotor
10000
4×500ml
16110
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Features
1. Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
2. Brushless frequency motor for model GL12 which in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. Model GL12 with the digital display which indicates the speed、time and temperature.
4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. There are high speed rotors and low speed rotors for your choice.
6. Electric lid lock, compact design, super speed and imbalance protection.
7. The centrifuge body is made of high-quality steel, safe and reliable.
t-Boc-aminooxy-PEG2-propargyl is a click chemistry crosslinker. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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t-Boc-aminooxy-PEG2-propargyl is a click chemistry crosslinker. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.
Gel images of different ranges of size selection. Sheared human genomic DNA was used as input.
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DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications. DNA size selection is preferred after NGS library prep in most of the cases. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.
Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.
DNA size selection with dual clean-ups.
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A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.
DNA size selection with single clean-up for >5 kb and >10 kb DNA.
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Features of DNA size selection and library size selection
High specificity and high recovery of size selection
11 selection ranges are available, including 5 ranges for NGS library size selection
50-100 bp
100-200 bp
200-500 bp
250-350 bp: ideal for illumina PE100 sequencing
300-450 bp: ideal for illumina PE150 sequencing
450-750 bp: ideal for illumina PE300 sequencing
500-1000 bp
1-3 kb
1-5 kb
>5 kb: ideal for long-read sequencing
>10 kb: ideal for long-read sequencing
Fast and simple
20-min protocol
No gel purification required
No columns required
No centrifugation required
Efficient removal of contaminants and unwanted components