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NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)

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The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition, all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible.

Detail

The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition,  all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of  hands-on time. Library multiplexing up to 12 samples is possible.

NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)

NGS DNA Fragmentation & Library Prep Kit Workflow

The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The protocol is optimized to generate libraries from 200 bp to 500 bp in size. The library size is inversely correlated with the incubation time of step 1 at 20°C.

Kit features:

      • Fast: 1-hour library construction from intact genomic DNA to NGS library
        • Total time: 1 hr
        • Hands-on time: 5 min
      • Intact genomic DNA can be used directly as input; DNA fragmentation is not required.
      • Works with both EDTA-free DNA samples and DNA samples in TE buffer
      • Simple workflow
        • Three steps
        • Only one beads purification step
      • Guaranteed quality: high library conversion efficiency based on our chemistry

NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)

Library conversion efficiency for NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform). 100 ng, 300 ng and 500 ng of DNA were used as input.

NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)

NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform): Library size distribution with different incubation time.

Other Products

OT-2 HEPA Module

The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.

The Opentrons HEPA Module comes in two different voltages. Your account manager will help you choose the correct one for your geographic region.

96S Super Magnet Plate

Permagen’s direct replacement magnet plate for either PN: A32782 & A001322. Comes with spring cushion base and same strength magnets to meet current protocol requirements.  49% Cost savings

For faster separations you can upgrade to our SKU# MSP500R which comes with all of the same features, just stronger magnets. 

Compatible with any magnetic beads & all protocols requiring a 96S super magnet plate

SKU #

A092722

Features

  • ANSI/ SBS footprint
  • Cushion base for added benefits
  • Held to OEM or better standards 
  • Precision manufactured for more consistent results
  • Fast magnetic bead separations
  • 4 mm bead free ring at bottom of each well for easy removal
  • Made in USA 
  • For Research use only

Compatibility

  • Round bottom plates 
  • Pyramid bottom plates 
  • PCR Plates
  • Volumes Up to 1000 µL volumes
  • Any magnetic beads
  • Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volume

Maximum – 2 mL 

Minimum – 30 µL

Propargyl-PEG3-Ms

Propargyl-PEG3-Ms is a crosslinking reagent with a propargyl group and a mesyl group. The propargyl group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.