The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
Detail
The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
NGS DNA Library Prep Kit Workflow
Kit features
Simple procedure: Reactions for all three steps are in one tube
Fast protocol
Total protocol time is around 1 hour
Hands-on time is only ~5 minutes
Guaranteed high library conversion efficiency as compared to other kits
Input DNA amount: from 50 ng to 1 ug
No insert concatemer ligation
Other Products
Sulfo DBCO-PEG4-amine
Product Info
Document
Product Info
Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
Features
Specifications
Recommended application
Plasmid Medium Yield preparation
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/B, 8 layers
Membrane aperture
1.0 μm
Maximum binding yield of plasmid
250 μg
Maximum yield of alcohol mediated Binding
1 mg
Plasmid Yields
Up to 0.25mg
Single liquid carrying capacity of column
4 ml
Minimum elution volume
500 μl
Withstand centrifugal force
5,000 x g
Centrifuge
Lowspeed centrifuge for 15ml centrifuge tubes, >3000 x g, swing-out Rotor, or Fixed Angle Rotor
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13121
HiPure DNA Midi Column III (8 x GF/B)with 15ml Collection Tubes
100/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
