NH-bis(PEG2-propargyl)

Overview

• Purification and enrichment of intact urinary exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• Versatile urine input volumes
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes are required
• No precipitation reagents nor overnight incubation required
• Compatible with urine from most species
• Pure exosomes are purified and are free from any other RNA-binding proteins
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome Purification and RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome Purification and RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome Purification and RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Details

Supporting Data

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Kit Specifications
Minimum Urine Input	250 μL
Maximum Urine Input	1 mL
Size of Exosomes Purified	40 nm – 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 – 40 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component	Cat. 58400 (50 preps)	Cat. 58700 (25 preps)	Cat. 58800 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	20 mL	20 mL	20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	18 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	50	25	15
Collection Tubes	50	25	15
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

DL6MB large capacity refrigerated centrifuge

Features:

1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.

2. Brushless frequency motor,  in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. Digital display which indicates the program, speed, time,RCF, temperature.

4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. There are 6 kinds of rotors for your choice.

6. Electric lid lock, compact design, super speed and imbalance protection.

7. The centrifuge body is made of high-quality steel, safe and reliable.

DL6MB Technical Parameter：

Max. Speed	6000rpm
Max. RCF	6600*g
Max. Capacity	6×1000ml(6*500ml blood bag）
Time Range	0~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 55
Temperature	-20-40 degrees
Acc/Dcc	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V  50HZ/60HZ
Size (W x D x Hmm)	800×675×830mm
Net Weight(Kg)	310KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for DL6MB

Order No.	Rotor No.	Max Speed (rpm)	Max Volume(ml)	Max.RCF（×g）
No30298	Swing Rotor	4200	6×1000ml	5100
NO30221	Angle Rotor	6000	4×300ml	5390
NO30222	Angle Rotor	6000	6×300ml	5660
NO30223	Angle Rotor	6000	6×500ml	6600

Widely used in the field of blood bank, pharmaceutical factory and laboratory.