NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.
Detail
NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.
Other Products
Blood DNA Isolation Kits
Product Info
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Product Info
Overview
High yield and high purity DNA ready for any application
Available in a variety of formats to properly suit your needs
Compatible with blood collected on a variety of commercially available tubes
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
The DM2300 ExcelBand™ 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2300 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 3,000 bp
Concentration
56 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months 4°C for 12 months -20°C for 36 months
Document
The DM2300 ExcelBand™ 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2300 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
SNPsig® VariPLEX SARSCoV-2 is a real-time-reverse transcriptase PCR (RT-PCR) multiplex assay to be used as a reflex qPCR test. VariPLEX™ SARS-CoV-2 can be performed on extracted sample eluates from positive COVID-19 tests.
Detects the variants originally identified in the UK (20I/501Y.V1), South Africa (20H/501Y.V2), Brazil (20J/501Y.V3) and California (20C/S:452R), and the key biologically significant mutations N501Y and E484K, which are now all prevalent globally.