The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
The performance of the reagents is second to none. We are confident that you will find excellent data quality and even see an improvement in data quality versus many traditional frozen master mixes.
Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.
Other Products
ssDNA Quantification Kit
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ssDNA Quantification Kit
The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
Our kit detects ssDNA by using fluorescent dye that enables sensitive single stranded DNA quantification , including ssDNA viruses, synthetic ssDNA, first-strand cDNA synthesis, denatured DNA, and bisulfate-converted DNA etc. ssDNA quantification is essential for the study of the biological process involves ssDNA.
Features
Optimized for use with the Qubit® Fluorometer
Uses the Qubit® ssDNA assay setting
Linear range: 1-200 ng ssDNA
Cost saving by more than 50%
A series of input ssDNA (200, 400, 600, 800, 1000, and 1200 ng) was used.
The performance of the BioDynami ssDNA Quantification Kit is nearly identical to that of Thermo Fisher’s Qubit ssDNA kit (figure below).
Comparison of BioDynami ssDNA Quantification Kit with Thermo Fisher kit.
Common contaminants such as salts, solvents, or detergents are well tolerated in the assay (Table 1).
Contaminants has been tested in BioDynami ssDNA Kit.
Document
The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
Plasmid Purification Magnetic Beads (RNA Depletion)
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Plasmid Purification Magnetic Beads (RNA Depletion)
Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.
We have developed a simple reagent to completely remove RNA contamination in the isolated plasmid samples using Solid Phase Reversible Immobilization (SPRI) beads. SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. Our Plasmid Purification Magnetic Beads (RNA Depletion) combines BioDynami’s proprietary chemistries with the reversible DNA-binding properties of SPRI magnetic beads. The reagent removes RNA and recovers the plasmid in the same step. Moreover, unwanted components such as salts, dNTPs, proteins, enzymes, and other impurities can also be removed simultaneously.
Plasmid can be used for downstream applications such as enzymatic digestion, transformation, transfection and molecular cloning etc. The beads can be an effective and inexpensive reagent for bacterial RNA depletion for routine plasmid purification.
Features
Effective depletion of bacterial RNA by RNase
High recovery rate of plasmid DNA by magnetic beads
Removal of unwanted components and impurities
Simple and fast beads-based protocol
Document
Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.
Fifteen discrete fragments ranging from 300 bp to 24000 bp
Higher intensity reference bands at 5000 bp and 10000 bp
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
UltraRanger 1kb DNA Ladder (Cat# 12100) – 100 loads
Ladder Properties: • Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
Fragment
Size (bp)
Mass (ng)
1
24000
30
2
14000
27
3
10000
60
4
8000
44
5
6000
36
6
5000
55
7
4000
49
8
3000
39
9
2500
35
10
2000
30
11
1500
33
12
1000
10
13
700
21
14
500
18
15
300
11
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
