Opentrons Flex Filter Tips, 1000 µL

• Format: 96-well microtiter plate (12 test strips of 8 wells)
• Standards: 0 | 0.15 | 0.4 | 1 | 2 | 5 ppb
• Incubation Time: 75 Minutes
• Compatible for use with US EPA Method 546

Description

Microcystin are a class of hepatotoxins produced by blue-green algae such as Microcystis aeruginosa.  Microcystin-LR is the most common of the over 50 different congeners. Cyanobacteria can produce microcystin in large quantities during an algae bloom which then pose a major threat.

This kit can be used for Congener-Independent quantitative test of Microcystin in liquid samples such as drinking, ambient and waste water and algal cultures.

Additional Quality Control, Calibration Verification, and Spiking Solution materials can be obtained separately in optional Supplemental Pack for Method 546 (catalog # EL2024-Q).

Microcystin-LR

• Informational sign postings about HABs at recreational waters: < 6 μg/L
• Recreational public health advisory: 6 μg/L
• Elevated recreational public health advisory (e.g. no contact): 20 μg/L

EPA 10-day drinking water health advisories:

• Do Not Drink – 0.3 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.
• Do Not Drink – 1.6 μg/L for school-age children to adults.
• Do not Use – 20 μg/L

Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.15 | 0.4 | 1 | 2 | 5 ppb
Incubation Time: 75 Minutes
Compatible for use with US EPA Method 546

Overview

Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.

• Heat sealing is a quick and cost effective method of plate sealing
• Our Gas PermASeal II Heat Seal is a paper with a grid lacquer coating which is porous and gas permeable
• The Gas PermeASeal II Heat Seal is compatible with polypropylene, polyethylene, polystyrene and cyclic olefin copolymer (COC) plates
• The seal can be removed by peeling, or it can be pierced with a pipette tip manually or using a liquid handling robot
• The Seal has a temperature range from -20°C to 80°C
• It can be used for insect and seed storage, as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in roll format compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.

• Real time qPCR kit
• For screening microcystin gene cluster
• Use in combination with Attogene Algae DNA isolation kit

Description

• Description

Attogene’s PCR kit for Microcystin is designed for the In vitro analysis of the MycE gene region responsible for assembling part of the Microcystis peptide. The MycE gene region specific primer and probe mix is provided to be detected through the FAM channel on a qPCR machine. A sample of algae is obtained and washed to extract a clean algal gDNA sample. A reaction mixture is assembled from primers, probe, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest; while the DNA probe mixture is cleaved during amplification to release its FAM fluorophore. The resulting FAM release can be detected on a variety of qPCR platforms.

Real time qPCR kit
For screening microcystin gene cluster
Use in combination with Attogene Algae DNA isolation kit