For scaling up and fully automating PCR setup and thermocycling.
With the Flex PCR Workstation, you can automate your PCR setup and thermocycling at the scale you need to increase efficiency, reduce errors, and save hands-on time. Configure your workstation with our on-deck Thermocycler for end-to-end automation, or with no Thermocycler for automated PCR setup with off-deck thermocycling.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex PCR Workstation*
Detail
For scaling up and fully automating PCR setup and thermocycling.
With the Flex PCR Workstation, you can automate your PCR setup and thermocycling at the scale you need to increase efficiency, reduce errors, and save hands-on time. Configure your workstation with our on-deck Thermocycler for end-to-end automation, or with no Thermocycler for automated PCR setup with off-deck thermocycling.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex PCR Workstation*
Other Products
C1410 MagPure Particles
Product Info
Document
Product Info
Introduction
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Details
Specifications
Features
Specifications
Concentration
100 mg/ml
Appearance
Suspension of black particles
Surface functional group
Si-OH, Silanol
Dispersibility
Polydisperse Amorphous
Particle size
1.5-5 μm
Preservation conditions
Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth.
Magnetic response speed
15-30 seconds
Settling velocity
>5 minutes
High salt mediated binding
>2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding
2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated binding
The recovery of DNA/RNA was up to 85%
DNase/RNase
Not detected
DNA residue
<1 ppm
Recommended application
Plasmid extraction, gel DNA recovery, genomic DNA extraction and RNA extraction.
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification
DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up
Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation
Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction
DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays
The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.
Document
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 250 µL to 30 mL
Urine Exosome Purification Mini Kit (250 µL – 1 mL urine)
Urine Exosome Purification Midi Kit (2 mL – 10 mL urine)
Urine Exosome Purification Maxi Kit (11 mL – 30 mL urine)
Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any protein-bound cell-free circulating RNA
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
NanoSight® Analysis
Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.
Exosomal RNA Analysis
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
Important Note Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
Attogene’s Microcystin Test Kit (Rapid – Lab) can be used to detect microcystins in liquid samples.
• Format: 10 tests
• Run Time: 10 Minutes
The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.
A tiered notification system which takes different actions based on different numeric thresholds for microcytin-LR concentrations in recreational waters has been developed. This is guidance that allows states to take various actions—such as posting information about harmful algal blooms (HABs), issuing a recreational public health advisory, or temporarily closing recreational waters through a no contact advisory—depending on the severity of the bloom event.
Microcystin-LR
Informational sign postings about HABs at recreational waters: < 6 μg/L Recreational public health advisory: 6 μg/L Elevated recreational public health advisory (e.g. no contact): 20 μg/L