For scaling up and fully automating PCR setup and thermocycling.
With the Flex PCR Workstation, you can automate your PCR setup and thermocycling at the scale you need to increase efficiency, reduce errors, and save hands-on time. Configure your workstation with our on-deck Thermocycler for end-to-end automation, or with no Thermocycler for automated PCR setup with off-deck thermocycling.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex PCR Workstation*
For scaling up and fully automating PCR setup and thermocycling.
With the Flex PCR Workstation, you can automate your PCR setup and thermocycling at the scale you need to increase efficiency, reduce errors, and save hands-on time. Configure your workstation with our on-deck Thermocycler for end-to-end automation, or with no Thermocycler for automated PCR setup with off-deck thermocycling.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex PCR Workstation*
TGW16 Technical Parameter：
| Max. Speed | 16000rpm |
| Max. RCF | 19040×g |
| Max. Capacity | 10×5ml |
| Time Range | 0~99min59s |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 55 |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 355×270×205mm |
| Net Weight(Kg) | 16KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TGW16
| Order No | Rotor | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| W16-1 | Angle rotor | 16000 | 40×0.2ml | 19040 |
| W16-2 | Angle Rotor | 16000 | 24×0.5ml | 18480 |
| W16-3 | Angle Rotor | 16000 | 12×1.5/2ml | 17940 |
| W16-4 | Angle Rotor | 16000 | 10x5ml | 17880 |
| W16-5 | Angle Rotor | 14000 | 20×1.5/2ml | 15580 |
| W16-6 | Angle Rotor | 14000 | 4x8PCR | 12070 |
1. Brushless motor, no pollution, free-maintenane.
2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting , operate simply.
3. Electric lid lock, super speed, imbalance protection.
4. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable.
5. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
6. 3 tiers protection steel cover and get the ideal centrifuation result.
Sample type purification kit guide
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Figure 1 / 3
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70100 (24 preps) | Cat. 70110, 70120, 70130, 70140 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V2 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
