OT-2 20µL Tips

Application

Digital incubator for the Gel cards. Blood card/ID card incubator holds 2 independent incubation zones with a capacity for 12 Gel cards, It can be suitable for many types of gel cards from different manufacturers.

Features

1. Keep 24-Gel Cards in temperature of 37 degree.
2. 2 independent incubation zones with a capacity for 12 Gel cards
3. Digital display to set up time and keep the temperature
4. Audible programmable alarm to notify end of incubation period

Technical Parameter

Ambient Temperature	10~37℃
Temperature uniformity	≤±0.5℃
Max. Capacity	24   Blood cards
Size (W x D x Hmm)	390×280×140mm
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Incubation   Temperature	37℃±0.1℃
Temperature Accuracy	<0.5℃
Rated   Power	250W
Net Weight	6.2KG
Time   Range	0~99min59sec
Certificates	CE,ISO   & Calibration report are available

Overview

• Detection kits for Streptococcus dysgalactiae
• Available in TaqMan format for analysis

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus dysgalactiae, a Lancefield group C α-hemolytic gram-positive bacteria, is a common pathogen in subclinical and clinical mastitis causing substantial economic losses in dairy herds. Streptococcus dysgalactiae has been isolated from infected mammary glands and teat injuries and is transmitted primarily during milking. However, detection of S. dysgalactiae in other cow tissues such as tonsils, mouth and vagina, and occurrence of S. dysgalactiae mastitis during the non-lactating period in herds, suggests that the organism also behaves as an environmental pathogen.

Streptococcus dysgalactiae TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Streptococcus dysgalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM30750 (100 preps)	Cat. TM30710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
S. dysgalactiae Primer & Probe Mix	280 μL	280 μL
S. dysgalactiae Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications

Features

• Aptamer-based hot start PCR
• Reversible enzyme inactivation
• Omits extra enzyme activation step
• Convenient for room temperature PCR set-up
• High yield and specificity of target amplicons
• Wide range of amplicon length (up to 10 kb)
• High sensitivity (as low as 1 fg of plasmid)

Applications 

• High specificity PCR
• Generation of PCR products for TA cloning
• Routine PCR, multiplex PCR, colony PCR, and RT-PCR

Storage

-20°C for 24 months 

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications