Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)

The kits were developed for plasmid miniprep directly from bacterial cultures. 100% centrifuge-free; NO vacuum needed; No column needed.

Plasmid MiniPrep Kit (Magnetic Beads), Cat.# 50012
– Use 2 ml of bacteria culture directly

Plasmid MiniPrep High Throughput Kit (Magnetic Beads), Cat.# 50011
– High throughput: Use 0.2 ml of bacteria culture directly with 96-well plates
– Low throughput: Use 0.2 ml of bacteria culture directly with 1.5 ml tubes

The kits can be used for both high copy and low copy numbers of plasmid DNA. With BioDynami’s proprietary magnetic beads technology, the kits eliminate the requirement of centrifuge, vacuum, and column. Our magnetic beads provide a robust and reliable tool for both high throughput and low throughput applications of plasmid DNA isolation with high binding capacity and fast magnetic response time.

The High Through kit (Cat.# 50011) can be used for both high throughput and low throughput extraction of plasmid DNA. Up to 96 samples can be extracted simultaneously when using a 96-well plate.

Yield of plasmid DNA may vary dependent on the bacteria strain, plasmid type, copy numbers, and growth conditions etc. DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, molecular cloning, restriction enzymatic digestion, transfection, and transformation etc. The isolated plasmid DNA with the magnetic beads is free of contaminations such as RNA, bacterial DNA, proteins, and other impurities.

Plasmid DNA were loaded on 1% of agarose gel after extraction.

Comparison of workflows of Magnetic Beads based kits

Features

• 100% centrifuge-free
  • • Culture medium can be used directly without centrifuge to pellet the bacteria
• Simple
  • • No centrifuge
    • No column
    • No vacuum
• Flexible applications with simplified miniprep protocol
  • • High throughput: 96-well plates with 0.2 ml of each culture (Cat.# 50011)
    • Low throughput: 1.5 ml tubes with 0.2 ml of each culture (Cat.# 50011)
    • Low throughput: 1.5 ml tubes with 2 ml of each culture (Cat.# 50012)
    • Ideal for screening and other applications

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions

Product Description

Isothermal Amplification Kit: Freeze-Dried Reagent, Stable & Easy to Operate

Product Detail

 Kit Storage and Term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months 

Isothermal Nucleic Acid Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Isothermal Nucleic Acid Applications

Suitable for DNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Colloidal gold probe:Require a sequence of 46-52nt in length

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.