Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
Detail
About
PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Ascorbic Acid
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
578
Signal Response:
Increase
Linear Range:
0.5 to 30 µg of L-ascorbic acid per assay
Limit of Detection:
0.175 mg/L
Reaction Time (min):
~ 8 min
Application examples:
Wine, beer, fruit juices, soft drinks, jam, milk, dairy products (e.g. cheese), dietetic foods, baby foods, processed meat, baking additives, fruit and vegetables (e.g. tomato and potato), pharmaceuticals, feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by MEBAK
The Ascorbic Acid (L-Ascorbate) assay kit is for the specific measurement and analysis of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
All reagents stable for > 6 months after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The Ascorbic Acid (L-Ascorbate) assay kit is for the specific measurement and analysis of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products.
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from FFPE tissue samples
Applications
PCR, southern blot and viral DNA detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples
Sample amount
<20mg
Elution volume
>15µl
Time per run
≤20 minutes
Liquid carrying volume per column
4ml
Binding yield of column
100μg
Principle
Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.
Advantages
Safety – deparaffinating without contacting with xylene or other toxic solution
Fast – without overnight incubation and digestion, several samples can be extracted within 2hours
High efficiency -remove the formaldehyde modification of DNA, greatly enhancing the sensitivity of PCR
Kit Contents
Contents
D312602
D312603
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Buffer DPS
30 ml
150 ml
Buffer ATL
15 ml
60 ml
Buffer AL
15 ml
60 ml
Buffer GW1*
22 ml
88 ml
Buffer GW2*
12 ml
50 ml
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer AE
10 ml
30 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Document
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Fast and easy recovery of DNA from agarose gel fragments
High recovery of desired DNA
Convenient spin column format
DNA is ready for ligation, restriction digestion, sequencing and more
This kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing. The protocol can be completed in 20 minutes.
