CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Parvovirus B19 was the first known human virus in the family of Parvoviruses. B19 is a non-enveloped, icosahedral virus that contains a single-stranded linear DNA genome. It is classified as an erythrovirus because of its capability to invade red blood cell precursors in the bone marrow. B19 virus causes a childhood rash called fifth disease or erythema infectiosum which is commonly called “slapped cheek” syndrome due to the appearance of erythema across the cheeks. The virus is primarily spread by infected respiratory droplets, but some cases of blood-borne transmission have been reported. Parvovirus infection in pregnant women is associated with hydrops fetalis due to severe fetal anemia, sometimes leading to miscarriage or stillbirth. The risk of fetal loss is about 10% if infection occurs before week 20 of pregnancy. There is also some evidence that intrauterine Parvovirus B19 infection leads to developmental abnormalities in childhood. Due to the serious nature of the virus, methods for its rapid diagnosis are desirable.
Parvo B19 TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Parvo B19 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component
Cat. TM39650 (100 preps)
Cat. TM39610 (100 preps)
MDx TaqMan 2X PCR Master Mix
2 x 700 μL
–
Parvovirus B19 Primer & Probe Mix
280 μL
280 μL
Parvovirus B19 Positive Control
150 μL
150 μL
Nuclease-Free Water (Negative Control)
1.25 mL
1.25 mL
Product Insert
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Other Products
D3125 HiPure DNA Micro Kit
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications
PCR, southern bolt and virus detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount
Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Advantages
Fast – several samples can be extracted in 20 minutes (after digestion)
High purity – purified DNA can be directly used in various downstream applications
High recovery – DNA can be recovered at the level of PG
Good repeatability – silica technology can obtain ideal results every time
Kit Contents
Contents
D312502
D312503
Purification Times
50 Preps
250 Preps
Buffer ATL
15 ml
60 ml
Buffer AL
15 ml
60 ml
Buffer GW1*
22 ml
66 ml
Buffer GW2*
20 ml
2 x 50 ml
Carrier RNA
310 μg
2 x 310 µg
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer AE
15 ml
60 ml
HiPure DNA Mini Columns I
50
2 x 125
2 ml Collection Tubes
100
5 x 100
Storage and Stability
Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Experiment Data
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 1-6ml plasma, serum, body fluids
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
1-6ml
Elution volume
≥50μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
High purity – OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0
Kit Contents
Contents
IVD5435
Purification Times
50
MagPure Particles F
14 ml
Carrier RNA
310 μg
Proteinase K
240 mg
Protease Dissolve Buffer
15 ml
Buffer SDS
15 ml
Buffer MLK
500 ml
Buffer MAW1
250 ml
Buffer MW2*
50 ml
Elution Buffer
60 ml
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Document
This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.
Staphylococcus Aureus PCR Pathogen Detection Kit For Isothermal Fluorescence Detector
Product Info
Document
Product Info
Product Description
Product Description:
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Amp-future bio, a leading Chinese manufacturer of detection kits, is proud to introduce its new Pathogen Detection Kit. This innovative swab test kit is specially designed for rapid detection of influenza and other infectious diseases, delivering results in as little as 5-20 minutes. Featuring a storage temperature of -20ºC, this test kit is highly reliable and easy to use. It is ideal for hospitals, medical centers, and other healthcare facilities to quickly and accurately detect pathogens. As a trusted name in the industry, Amp-future bio guarantees the highest quality and safety when it comes to its Pathogen Detection Kit, making it the perfect choice for all your rapid test kits and infectious disease test kits needs.
Support and Services:
Pathogen Detection Kit technical support and services are offered to customers to ensure optimal performance and usability of the product. Technical support is available through our customer service hotline as well as our online customer portal. Our technical support team is available to answer any questions and provide guidance for product installation, troubleshooting, and maintenance. Additionally, our team provides software updates and upgrades, as well as access to our library of technical documents and resources. We also offer training and education services for customers who wish to better understand the product and its capabilities.
Packing and Shipping:
To ensure that our Pathogen Detection Kit arrives to you in perfect condition, we take special care in packaging and shipping.
Before shipping, all kits are carefully inspected for product quality. They are then bubble-wrapped and sealed in a box to provide the highest level of protection.
We use a reputable shipping company with tracking options and signature confirmation. Depending on the shipping destination, the package may require additional documentation, such as a Certificate of Origin or an invoice.
We strive to deliver your order as quickly and cost-effectively as possible. We offer same day shipping for orders placed before noon EST.
FAQ:Q1: What is Amp-future bio Pathogen Detection Kit?A1: Amp-future bio Pathogen Detection Kit is a high-performance product developed by China for rapid detection of pathogenic microorganisms.Q2: What microorganisms can be detected by this kit?A2: The Amp-future bio Pathogen Detection Kit can detect a wide range of pathogenic microorganisms, including bacteria, viruses, and parasites.Q3:Is the kit easy to use?A3: Yes. The Amp-future bio Pathogen Detection Kit is designed for easy operation and use. It comes with detailed instructions for use.Q4: Is the kit safe?A4: All components of the Amp-future bio Pathogen Detection Kit are strictly tested to ensure safety. The kit is safe and reliable to use.
Document
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
