PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
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| Kit Specifications | |
| Maximum DNA Input | 25 μg |
| Final Endotoxin Level | ≤ 0.1 EU/µg DNA |
| Size of DNA Purified | Up to 13,000 bp |
| Maximum DNA Volume Input | 100 μL |
| Average Recovery | > 90% |
| Time to Complete 10 Purifications | 20 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 22700 (25 preps) | Cat. 52200 (10 preps) | Cat. 21900 (4 preps) |
|---|---|---|---|
| Buffer SK | 15 mL | 60 mL | 60 mL |
| Wash Solution H | 18 mL | 18 mL | 18 mL |
| Elution Buffer I | 6 mL | 12 mL | 12 mL |
| Endotoxin Removal Solution | 1.5 mL | 1.5 mL | 1.5 mL |
| Precipitation Solution | – | 1.5 mL | 1.5 mL |
| Spin Columns (assembled with Collection Tubes) | – | 10 | 4 |
| Spin Columns | 25 | – | – |
| Collection Tubes | 25 | – | – |
| Elution Tubes (1.7 mL) | 25 | – | – |
| Elution Tubes (15 mL) | – | 10 | – |
| Elution Tubes (50 mL) | – | – | 4 |
| Product Insert | 1 | 1 | 1 |
2 Layer Cell Factory Double Wide Mouth
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
PRODUCT FEATURES
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
10 Layer Cell Factory Double Wide Mouth
Huayi Cell Factory is a robust and easy-to-use cell
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
PRODUCT FEATURES
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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