Isolate high quality DNA from a broad variety of phage strains
High yields of total DNA
Fast and easy processing using a rapid spin-column format
No phenol or chloroform extractions or cesium chloride banding required
High yields of DNA recovered3-15 µg DNA from 106-1010 pfu/ mL of enriched phages
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
3-15 µg DNA from 106-1010 pfu/mL of enriched phages
Time to Complete 10 Purifications
45 minutes
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component
Cat. 46800 (50 preps)
Cat. 46850 (100 preps)
Lysis Buffer B
40 mL
2 x 40 mL
Wash Solution A
38 mL
2 x 38 mL
Elution Buffer B
8 mL
2 x 8 mL
Spin Columns
50
100
Collection Tubes
50
100
Elution Tubes (1.7 mL)
50
100
Product Insert
1
1
Other Products
Large Capacity Refrigerated Centrifuge
Product Info
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Product Info
DL6MB Technical Parameter：
Max. Speed
6000rpm
Max. RCF
6600×g
Max. Capacity
6×1000ml
Timer
1~9h59min
RPM/RCF Convert
Yes
Noise (dB)
≤ 60
Temperature Range
-20~40℃
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V 50HZ/60HZ
Size (L x W x Hmm)
800×740×930mm
Net Weight(Kg)
310KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for DL6MB
Order No.
Rotor
Max Speed (rpm)
Max Volume(ml)
Max.RCF（×g）
6MB-1
Swing Rotor
4200
6×1000ml
5100
6MB-2
Angle Rotor
6000
4×300ml
5390
6MB-3
Angle Rotor
6000
6×300ml
5660
6MB-4
Angle Rotor
6000
6×500ml
6600
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Features
1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.
2. Frequency conversion motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. With pre-cooling function,Digital display which indicates the program, speed, time,RCF, temperature.
4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. There are several kinds of rotors for your choice.
6. Electric lid lock, compact design, super speed and imbalance protection.
7. The centrifuge body is made of high-quality steel, safe and reliable.
Usages: For the differentiation of Gram-negative bacteria on the basis of citrate utilization.
Principle: Magnesium ions in various metabolic cofactors; ammonium dihydrogen phosphate to provide nitrogen; dipotassium hydrogen phosphate is the buffer; sodium citrate as a carbon source; agar as medium coagulant.
Formulation(per liter): Sodium chloride 5g Magnesium sulfate 0.2g Ammonium dihydrogen phosphate 0.2g Sodium ammonium phosphate 0.8g Sodium citrate 2g Agar 15g Bromothymol blue 0.08g Final pH 7.0 ± 0.2
How to use: 1.Suspend 23.3g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Urine Exosome and Free-Circulating RNA Isolation Kits
Product Info
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Product Info
Overview
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
Versatile sample input ranges
Isolate all sizes of free-circulating RNA, including microRNA
Bind and elute all RNA irrespective of size or GC content, without bias
The purified exosomal RNA is free from any circulating RNA-binding proteins
No phenol extractions, Proteinase K treatment nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes are required
No precipitation reagents nor overnight incubation required
Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Urine Exosome and Free-Circulating RNA Isolation Mini Kit
For sample volumes ranging from 250 µL to 1 mL.
Urine Exosome and Free-Circulating RNA Isolation Midi Kit
For sample volumes ranging from 2 mL to 10 mL.
Urine Exosome and Free-Circulating RNA Isolation Maxi Kit
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50 – 100 µL
Time to Complete 10 Purifications
40 – 45 minutes
Average Yields*
Variable depending on the specimen
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipping. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
