The Plant RNA Kit (NA2014-01) is optimized for purification of total RNA from a wide variety of plant samples which rich in polysaccharides and phenol. It comes with proprietary magnetic beads and a specially optimized buffer. Toxic solvents such as phenol, chloroform are not needed in the process. The eluted RNA is high-quality and is ready for all downstream applications, such as RT-PCR, Northern blotting, differential display. The procedures can be fully automated on the magnetic particle processor instrument and ease of use.
Other Products
1.5 ml – 2 mL, 24- Tube Chiller Block
Product Info
Document
Product Info
1.5 mL – 2 mL Micro-centrifuge Tube or Screw-Cap Vial Chiller Block
SKU: CB024
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
Document
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate
Applications
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method
96 well Bind Plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
PCR product, enzymatic reaction
Sample amount
Appropriate
Recovery
≥80%
Elution volume
≥75μl
Time per run
≤60 minutes
Liquid carrying volume per column
800µl
Binding yield of column
20µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – ≥80% DNA recovery
Fast – isolation can be completed in 60 minutes
Kit Contents
Contents
D212201
D212202
D212203
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
Buffer GDP
120 ml
400 ml
4 x 400 ml
Buffer DW2
20 ml
100 ml
3 x 100 ml
Elution Buffer
20 ml
20 ml
30 ml
HiPure DNA Plate
1
4
20
2.2ml Collection Plate
1
4
20
1.6 ml Collection Plate
1
4
20
0.8 ml Collection Plate
1
4
20
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Document
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
