The Plant RNA Kit (NA2014-01) is optimized for purification of total RNA from a wide variety of plant samples which rich in polysaccharides and phenol. It comes with proprietary magnetic beads and a specially optimized buffer. Toxic solvents such as phenol, chloroform are not needed in the process. The eluted RNA is high-quality and is ready for all downstream applications, such as RT-PCR, Northern blotting, differential display. The procedures can be fully automated on the magnetic particle processor instrument and ease of use.
Other Products
High Efficiency RNA Isothermal PCR Kit Stable Easy Operate
Product Info
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Product Info
Product Description
High-Efficiency Isothermal RNA Amplification Kit, Stable And Easy To Operate
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
RNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA from skin, muscle, connective tissue, fibrous tissue sample
Applications
PCR and southern blot, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cultured cells and tissue samples
Sample amount
Tissue: < 20mg
Principle
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R511402
R511403
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RL
30 ml
150 ml
RNA Digestion Buffer
15 ml
80 ml
Buffer DW1
30 ml
150 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Buffer AE
10 ml
50 ml
Storage and Stability
HiPure DNA/RNA Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
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This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt is a crosslinker consisting of an amino group with two propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt is a crosslinker consisting of an amino group with two propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.