IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
Key features
IsoPol® BST+ lacks 5’-3’- and 3’-5’-exonuclease activity.
Inactivation – IsoPol® BST+ is completely inactivated after one minute incubation at 95°C
Enhanced strand displacement and processivity.
Increased salt tolerance.
IsoPol® BST+ Glycerol FREE
Is the popular choice f or isothermal applications such as LAMP and RT-LAMP at point-of-care diagnostics for its superior amplification performance and robustness. The enzyme is offered in a 1X storage buffer at a concentration < 300 U/µl
IsoPol® BST+ High Concentration Glycerol FREE
(IsoPol® BST+ HC Glycerol FREE) is used to ease the product development processes in sequencing technologies, solid phase amplification and several isothermal technologies. Same performance as IsoPol® BST+ (i.e., high processivity, relative SDA, and relative activity) with the only difference that it is offered in a 2X storage buffer at a concentration > 500 U/
Key features
High concentration
High purity
Lyophilisation and automation friendly
Figures
Properties
Document
These DNA polymerases are ideal for Isothermal Amplification
IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal Nucleic Acid Principle Summary
The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.
Isothermal Nucleic Acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal Nucleic Acid Applications
Suitable for DNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Colloidal gold probe:Require a sequence of 46-52nt in length
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.
Contents: 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
PCR Sizer 100 bp DNA Ladder (Cat# 11400) – 100 loads
Ladder Properties: • Ten discrete bands, ranging from 100 bp to 1,000 bp • Higher intensity band at 500 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
1000
78
2
900
70
3
800
62
4
700
55
5
600
47
6
500
78
7
400
31
8
300
24
9
200
31
10
100
24
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
