Overview

• Sample collection is non-invasive and painless
• Fast and easy high throughput processing using 96-well plates
• Isolate high quality genomic DNA
• Effective removal of PCR inhibitors
• Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

This kit features a rapid, high-throughput 96-well format for isolating high yield and high quality DNA from saliva.  The purified saliva DNA is compatible with any number of downstream applications including PCR, qPCR, Southern Blot analysis, sequencing, microarray analysis and more. This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000).  It is fully compatible to both vacuum and centrifugation protocols.

​Background

Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.

Details

Supporting Data

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Kit Specifications
Binding Capactity Per Well	50 μg
Maximum Loading Volume Per Well	600 μL
Size of DNA Purified	All sizes
Time to Complete 96 Purifications	40 minutes

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component	Cat. RU35200 (192 preps)
Lysis Buffer F	100 mL
Proteinase K in Storage Buffer	4 mL
Binding Buffer B	40 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Plate	2
96-Well Collection Plate	2
Adhesive Tape	4
96-Well Elution Plate	2
Product Insert	1

endo-BCN-pentanoic acid is a click chemistry reagent containing a BCN group and a terminal carboxylic acid. The BCN group can react with azide-tagged compounds or biomolecules. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-pentanoic acid is a click chemistry reagent containing a BCN group and a terminal carboxylic acid. The BCN group can react with azide-tagged compounds or biomolecules. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional plasmid, plasmid≤30KB
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤80 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – it takes only 80 minutes to complete the isolation
• High yield – up to 15μg plasmid can be binded in one column
• Economic – excellent cost effectiveness performance

Kit Contents

Contents	P181402	P181403	P181404
Purification Times	100 Preps	500 Preps	5000 Preps
RNase A	10 mg	30 mg	2 x 160 mg
Buffer P1	30 ml	150 ml	2 x 800 ml
Buffer P2	30 ml	150 ml	2 x 800 ml
Buffer LEN3	20 ml	80 ml	800 ml
Buffer LN4	90 ml	400 ml	4 x 980 ml
MagPure Particles	3.5 ml	17 ml	3 x 60 ml

Storage and Stability

RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature whenused. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.