Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format)

Overview

Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma and serum input volumes
Concentrate circulating and exosomal RNA into small elution volumes
Isolate inhibitor-free circulating and exosomal RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Available in Spin Column or 96-well plate formats
Process 96-well plates using vacuum or centrifugation
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Compatible with Streck Cell-Free RNA BCT® Tubes
Purification is based on Norgen’s proprietary resin matrix

These kits are able to isolate all sizes of circulating and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR. The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL.

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL.

Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (High Throughput Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation.

Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from various amounts of plasma/serum ranging from 2 mL to 5 mL.

Details

Supporting Data

Figure 1 / 5

Figure 1. Isolation and Detection of Circulating RNA from Different Plasma Volumes

Figure 2. Effective Isolation of Plasma Circulating RNA from Different Volumes

Figure 4. Isolation and Detection of Circulating RNA from Different Plasma Volumes

Figure 5. Effective Isolation of Plasma Circulating RNA from Different Volumes

Figure 6. Isolation and Detection of Circulating RNA from 1 mL Samples of Serum and Plasma

Click for expanded view

Kit Specifications – 96-well
Minimum Plasma/Serum Input	0.25 mL
Maximum Plasma/Serum Input	2 mL
Size of RNA Purified	All sizes, including microRNA
Time to Complete Purification	< 1 hour

Cat.29500 Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 51000 (50 preps)	Cat. 29500 (96 preps)	Cat. 42800 (50 preps)	Cat. 50900 (25 preps)
Slurry C1	–	–	–	6 mL
Slurry C2	12 mL	–	12 mL	–
Slurry C3	–	20 mL	–	–
Lysis Buffer A	2 x 130 mL	4 x 100 mL	2 x 130 mL	2 x 130 mL
Wash Solution A	38 mL	2 x 38 mL	38 mL	18 mL
Elution Solution A	6 mL	20 mL	6 mL	6 mL
Mini Filter Spin Columns	50	–	50	25
96-Well Filter Plate	–	1	–	–
Adhesive Tape	–	1	–	–
Collection Tubes	50	–	50	25
96-Well Collection Plate	–	1	–	–
Elution Tubes (1.7 mL)	50	–	50	25
96-Well Elution Plate	–	1	–	–
Product Insert	1	1	1	1

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Maximum Amount of Starting Material
Animal Cells	1 x 10⁶ cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 10⁶ cells)	15μg
E. coli Cells (1 x 10⁹ cells)	50 μg

Fragment	Size (bp)	Mass (ng)
1	10000	58
2	8000	53
3	6000	46
4	5000	67
5	4000	56
6	3000	47
7	2500	41
8	2000	34
9	1500	29
10	1000	18
11	700	13
12	500	23
13	300	14

Features	Specifications
Main Functions	Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	1mg

Contents	IVD3182
Purification Times	50
Buffer ACL	250 ml
Buffer ACB*	300 ml
Buffer DCW1*	22 ml
Buffer DCW2*	10 ml
Proteinase K	540 mg
Protease Dissolve Buffer	30 ml
Carrier RNA	110 μg
Nuclease Free Water	20 ml
HiPure CFDNA Mini Columns	50
2 ml Collection Tubes	100
Extender Tube	50
Vac-Connector	50

Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format)

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Supporting Data

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Component	Cat. 17200 (50 preps)	Cat. 37500 (100 preps)	Cat. 17250 (250 preps)	Cat. 17270 (500 preps)	Cat. 24300 (192 preps)	Cat. 24370 (576 preps)	Cat. 24350 (192 preps)	Cat. 24380 (576 preps)
Buffer RL	40 mL	2 x 40 mL	175 mL	350 mL	2 x 40 mL	350 mL	2 x 40 mL	350 mL
Wash Solution A	38 mL	2 x 38 mL	148 mL	1 x 148 mL 1 x 74 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL
Elution Solution A	6 mL	2 x 6 mL	30 mL	60 mL	2 x 20 mL	60 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	100	250	500	–	–	–	–
96-Well Isolation Plate	–	–	–	–	2	6	–	–
96-Well Isolation Plate (Deep Well)	–	–	–	–	–	–	2	6
Adhesive Tape	–	–	–	–	4	12	4	12
Collection Tubes	50	100	250	500	–	–
96-Well Collection Plate	–	–	–	–	2	6	–	–
96-Well Collection Plate (Deep Well)	–	–	–	–	–	–	2	6
Elution Tubes (1.7 mL)	50	100	250	500	–	–
96-Well Elution Plate	–	–	–	–	2	6	–	–
96-Well Elution Plate (Deep Well)	–	–	–	–	–	–	2	6
Product Insert	1	1	1	1	1	1	1	1