Description

As this is a 2 gene kit, we recommend purchase of 2 of the accompanying RT-qPCR master mix reagent: oasig Lyophilised OneStep RT-qPCR Master Mix 150 reactions.

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Overview

• Sample collection is non-invasive and painless
• Fast and easy processing using a magnetic bead system
• Isolate high quality genomic DNA
• This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices
• Also available in a 96-well format that can be integrated with a robotic automation system

Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva.  Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	2-5 μg
Average Purity (OD260/280)	1.6 – 1.8
Time to Complete 10 Purifications	30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. RU55400 (50 preps)	Cat. RU62900 (192 preps)
Lysis Buffer F	30 mL	2 x 30 mL
Proteinase K in Storage Buffer	1.2 mL	4 mL
Magnetic Bead Suspension	2 x 1.1 mL	8.5 mL
Elution Buffer B	8 mL	30 mL
Elution Tubes (1.7 mL)	50	–
96-Well Plate	–	2
96-Well Elution Plate	–	2
Adhesive Tape	–	2
Product Insert	1	1

Propargyl-PEG2-methylamine is a crosslinker with a propargyl group and methylamine group. The propargyl group forms triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG2-methylamine is a crosslinker with a propargyl group and methylamine group. The propargyl group forms triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.