ProbeSure Multiplex Master Mix is an enhanced version of ProbeSure Master Mix, formulated to enable users to analyse up to four targets in one reaction well. For example, two bi-allelic SNPs or one reference gene and a further three genes of interest.
Users will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ATTO 633 (the wavelengths of each of these can be found in our ProbeSure Multiplex Master Mix User Guide). ProbeSure Multiplex Master Mix is supplied at 2x concentration for convenience and is supplied with the ATTO 633 normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ATTO 633.
96-Well Low Elution PCR side pull bar magnetic plate with integrated cushion base
Product Info
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Product Info
Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
