Propargyl-PEG1-t-butyl ester has a propargyl group and a t-butyl protected carboxyl group. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG1-t-butyl ester has a propargyl group and a t-butyl protected carboxyl group. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Saliva Exosome Collection and Preservation Kit is an all-in-one solution designed for 1) simple and non-invasive saliva collection; 2) preservation of exosomes in saliva samples at ambient temperature; and 3) isolation of high quality intact exosomes and exosomal RNA within a laboratory setting. The Saliva Exosome Collection, Preservation and Isolation Kit contains 50 Individual Saliva Exosome Collection and Preservation Devices. Each of the 50 individual Saliva Exosome Collection and Preservation Devices consists of 3 components:
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed, the Collection Tube is securely capped with Collection Tube Cap. The collected saliva is mixed by gentle inversion for several times till the saliva colour changes into yellow. The Saliva Collection Tube is subsequently sent to the laboratory for the purification of intact exosomes and for the subsequent isolation and analysis of saliva exosomal RNA. The saliva exosomes in preserved samples are stable for 2 years at room temperature.
| Kit Specifications | |
| Volume of Saliva Collected | 2 mL |
| Preservation Temperature | Room Temperature |
| Preservation time | 2 years at room temperature |
Shelf Life and Handling
| Kit Components | Cat. 65400 (50 Devices) |
|---|---|
| Individual Saliva Exosome Collection and Preservation Devices | 50 |
| Donor Procedure Flowchart | 1 |
| Product Insert | 1 |
| Individual Saliva Exosome Collection and Preservation Device Contents | |
| Saliva Collection Funnel and Collection Tube | 1 |
| Collection Tube Cap | 1 |
| Donor Instructions | 1 |
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1 fg/μL, 0.3 fg/μL, 0.5 fg/μL, 1 fg/μL and 3 fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentrati on values of samples with 0.3 fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
