Propargyl-PEG1-t-butyl ester has a propargyl group and a t-butyl protected carboxyl group. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG1-t-butyl ester has a propargyl group and a t-butyl protected carboxyl group. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
P1814 MagPure Plasmid EF Mini Kit
Product Info
Document
Product Info
Introduction
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture
Applications
Enzyme digestion, sequencing, PCR and labeling, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Conventional plasmid, plasmid≤30KB
Sample amount
1-5ml
Elution volume
≥50μl
Time per run
≤80 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – it takes only 80 minutes to complete the isolation
High yield – up to 15μg plasmid can be binded in one column
RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature whenused. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at
Document
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Permagen’s 15 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 15 mL Centrifuge tubes
Accommodates any common 15 mL Centrifuge Tubes
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
This ELISA kit is intended for the quantitative detection of IgG antibodies against Leishmania infantum in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Leishmania infantum antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
Document
12 x 8 (96 tests) This ELISA kit is intended for the quantitative detection of IgG antibodies against Leishmania infantum in human serum.