Propargyl-PEG12-amine

Product Description

Amp-future DNA Isothermal Amplification Kit NFO: Freeze-Dried Reagent

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

TDL5B refrigerated low speed centrifuge

Technical Parameters

Max speed：	5000rpm	Max RCF：	4730xg
Max volume	4x800ml	Noise：	≤65dBA
Timer	1～99h59min	Net weight：	125 Kg
Dimension(HxDxW)：	430×688×715mm	Power supply	AC 220V 50HZ 15A
Temperature range	-20℃~40℃	Temperature accuracy	±1℃
Speed accuracy：	±20rpm	Package	wooden box

Matched Rotors for TDL5B

Order No.	Model	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
No.30671	Swing out rotor	4000	4x800ml (round cup)	3450
Adapters for 4x800ml (round cup) swing rotor for 5ml, 7ml, 10ml, 15ml, 50ml, 100ml, 250ml, 500ml tubes
No.30696	Swing out rotor	4000	4x500ml (square cup)	3310
No.31277	Swing out rotor body
	Matched Buckets for the swing out rotor body	5000	4x50ml	4420
			4x100ml
		4000	8x50ml	2950
			16x15ml
			24x15ml
		4000	24×10/7ml vacuum tube	2580
			16x10ml vacuum tube

Order No.	Model	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
No.31377	Matched Buckets for the swing out rotor body	5000	32x10ml vacuum tube	2580
			32x7ml vacuum tube adapter
			32x5ml vacuum tube adapter
			24x10ml vacuum tube	2580
			24x7ml vacuum tube adapter
			24x5ml vacuum tube adapter
			16x10ml vacuum tube	2580
			16x7ml vacuum tube adapter
			16x5ml vacuum tube ada