Propargyl-PEG24-amine

Overview

• Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
• Time Savings – Set up RT reactions in a shorter time since less pipetting steps are required
• Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
• High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
• Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility

TruScript Reverse Transcriptase is Available in Three Convenient Formats:

1. TruScript Reverse Transcriptase Kit (Cat.#54440)

This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.

2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.

3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.

Which TruScript Kit is Best for Your Needs?

	TruScript Reverse Transcriptase	TruScript First Strand cDNA Synthesis Kit for mRNA	TruScript First Strand cDNA Synthesis Kit
Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components)
Your template is enriched mRNA
Your template is total RNA (poly A OR non-poly A-containing transcripts)
Kit contains oligo dT
Kit contains both oligo-dT primer and Random Hexamers
Kit contains reaction buffer with nucleotides and primer		with oligo-dT	with oligo-dT and random hexamers
You have your own first strand synthesis primer
Your template is microRNA (using your own primers)
	Cat.#54440	Cat.#54400	Cat.#54420

Details

Supporting Data

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TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) – Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water 
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 54440 (10,000 units)	Cat. 54420 (50 rxns)	Cat. 54410 (50 rxns)
TruScript™ Reverse Transcriptase (200 units/ μL)	50 μL	–	–
5x RT Buffer	1 mL	–	–
TruScript™ Enzyme Mix	–	100 μL	100 μL
2x Reaction Mix	–	500 μL	–
2x Reaction Mix for mRNA	–	–	500 μL
Nuclease-Free Water	1.25 mL	1.25 mL	1.25 mL
Product Insert	1	1	1

DBCO-PEG12-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG12-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description 

The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Two reference bands — 75 kDa (red) and 25 kDa (green)

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control

Under suggested conditions, PM2700 ExcelBand™ 3-color Broad Range Protein Marker resolves 13 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage

The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.