t-Boc-N-Amido-PEG5-propargyl comprises a propargyl group and Boc-protected amine. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc group can be deprotected under mild acidic conditions to form the free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG5-propargyl comprises a propargyl group and Boc-protected amine. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc group can be deprotected under mild acidic conditions to form the free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-MRH2O2
SKU: 700004322
1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
| Content: | 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Hydrogen Peroxide |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance, Fluorescence |
| Wavelength (nm): | (Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570 |
| Signal Response: | Increase |
| Linear Range: | (Fluorescence) 0.0034-0.17 µg of H2O2 per assay (0.1-5 µM in-assay) (Absorbance) 0.034-0.68 µg of H2O2 per assay (1-20 µM in-assay) |
| Limit of Detection: | (Fluorescence) 0.0034 ug/mL (Absorbance) 0.25 ug/mL |
| Reproducibility (%): | (Fluorescence) ~ 8% (Absorbance) ~ 8% |
| Reaction Time (min): | (Fluorescence) 30 min (Absorbance) 30 min |
| Application examples: | Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.). |
This product has been discontinued.
The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.
Display all assay test kits available.
Fluorometric/UV method for the determination of H2O2 content in a variety of samples.
Advantages
The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.
Description
The DM3200 ExcelBand™ 1 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM3200 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 10,000 bp
Concentration
87 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
The DM3200 ExcelBand™ 1 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM3200 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
