Propargyl-PEG3-CH2CO2H is an PEG linker with an alkyne and carboxylic acid set of functional groups. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed.
Propargyl-PEG3-CH2CO2H is an PEG linker with an alkyne and carboxylic acid set of functional groups. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Figure 1 / 4
Click for expanded view
| Kit Specifications | |
| Maximum Column Binding Capacity | 50 μg for RNA 20 μg for DNA 200 μg for protein |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Size of DNA Purified | ≥ 30 kb |
| Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues | 5 x 106 cells 25 mg (for selected tissues) 100 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg |
| Time to Complete 10 Purifications | 30 minutes |
| Average Yield:HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells) | 15 μg RNA8 μg DNA150 μg protein |
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 47700 (50 preps) | Cat. 51600 (50 preps) | Cat. 51700 (96 preps) |
|---|---|---|---|
| Buffer SKP | 40 mL | 40 mL | – |
| Lysis Buffer Q | – | – | 40 mL |
| Wash Solution A | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL |
| Elution Buffer F | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution C | 30 mL | 30 mL | 60 mL |
| Binding Buffer A | 8 mL | 8 mL | 8 mL |
| Elution Buffer C | 8 mL | 8 mL | 30 mL |
| Protein Neutralizer | 4 mL | 4 mL | 4 mL |
| Protein Loading Dye | 2 mL | 2 mL | 3 x 2 mL |
| gDNA Purification Columns | 50 | – | – |
| gDNA Purification Micro Columns | – | 50 | – |
| gDNA Purification 96-Well Plate | – | – | 1 |
| RNA/Protein Purification Columns | 50 | – | – |
| RNA/Protein Purification Micro Columns | – | 50 | – |
| RNA/Protein Purification 96-Well Plate | – | – | 1 |
| Collection Tubes | 150 | 150 | – |
| Collection Plate | – | – | 5 |
| Elution Tubes (1.7 mL) | 150 | 150 | – |
| Elution Plate | – | – | 3 |
| Lysis Preparation Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Product Insert | 1 | 1 | 1 |
Description
The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program.
Features
Storage
Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)
Protect from light
-20°C for 12 months
The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program.
