Propargyl-PEG3-t-butyl ester consists of an alkyne group and a t-butyl ester group. The alkyne groupreacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG3-t-butyl ester consists of an alkyne group and a t-butyl ester group. The alkyne groupreacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method.
MUG permits the rapid detection of Escherichia coli when the medium is observed for fluorescence using a UV light. MUG is hydrolyzed by the enzyme β-glucuronidase which is produced by E. coli to yield a fluorescent end product 4- methylumbelliferone. Trypticase provides the essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentative action. The bile salts inhibit gram-positive bacteria especially the Bacillus species and faecal Streptococci.
| Ingredients | /liter |
| Trypticase or tryptose | 20 g |
| Bile salts No. 3 | 1.5 g |
| Lactose | 5 g |
| K2HPO4 | 4 g |
| KH2PO4 | 1.5 g |
| NaCl | 5 g |
| 4-methylumbelliferyl-β-D-glucuronide (MUG) | 50mg |
| pH6.9±0.2 at 25°C | |
Weigh 37g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until
completely dissolved, sterilize at 115℃ for 20min, cool to room temperature and set aside.
The following quality control strains were inoculated and cultured at 44.5℃±0.5℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Escherichia coli ATCC25922 | Blue-white fluorescence is produced under 366nm UV light |
| Salmonella typhimurium ATCC14028 | No fluorescence under 366nm UV light |
| Enterococcus faecalis ATCC29212 | Clear, no fluorescence |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method. Principle and Interpretation MUG permits the rapid detec……
The Opentrons 24-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block acts as a 24 position rack to keep 2mL tubes, 1.5mL tubes, and the NEST 0.5mL tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
The Opentrons 24-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block acts as a 24 position rack to keep 2mL tubes, 1.5mL tubes, and the NEST 0.5mL tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
Techinical parameter
| ltem No | SH7006 | SH9006 | SH9009 |
| Ice condenser capacity | 6kg | 6kg | 9kg |
| Ice condenser temperature | -70℃ | -90℃ | -90℃ |
| Chamber volume | 12L | 15L | |
| Freeze dried chamber | Square cavity/front door opening/door opening angle up to 180 degrees | ||
| Sample shelf | ·Stainless steel template partition● Electric heating/optional | ||
| Vacuum pump | Extreme vacuum5*10mbar ,with a pumping capacity of 8m³/hbuilt-in to the host/optional for various types of pumps | ||
| Oil mist filter | Standard configuration | ||
| Vacuum pump connection pipe | Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents | ||
| Anti-corrosive treatment | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | ||
| Fxternal valve | 12 ports/optional | 1-48 ports/optiona | |
| Eutectic point detection system | Support/Optional | ||
| Data export analysis | Support/Optional | ||
| Power | 0.95kw | 1.65kw | |
| Size | 700*580*1600mm | ||
Model:
SH7006,SH9006,SH9009
Brand:
Yingtai
