Propargyl-PEG4-bromide

K-CellG5-4V

SKU: 700004272

Content:	(K-CellG5-4V) 120 / 240 assays (manual) / 480 assays (auto-analyser) or  (K-CellG5-2V) 60 / 120 assays (manual) / 240 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	endo-Cellulase
Assay Format:	Spectrophotometer, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	1.2 x 10-3 U/mL
Reproducibility (%):	~ 3%
Total Assay Time:	10 min
Application examples:	Fermentation broths, industrial enzyme preparations and biofuels research.
Method recognition:	Novel method

The K-CellG5-2V pack size has been discontinued (read more).

Cellulase Activity Assay Kit.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

View Cellulase Activity Assay Protocol.

View our complete list of assay kits for enzyme activities.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

Description

As this is a 2 gene kit, we recommend purchase of 2 of the accompanying RT-qPCR master mix reagent: oasig Lyophilised OneStep RT-qPCR Master Mix 150 reactions.

Norovirus is known to cause acute gastroenteritis. It is a small (27-38 nm), round, nonenveloped RNA virus belonging to the Caliciviridae family and is responsible for over 80% of non-bacterial outbreaks of gastroenteritis in the world. It affects individuals of all ages, with a distinct seasonal link to winter. It has a genome of 7.6 kb that is positive sense and has a single stranded linear confirmation. It encodes a major structural protein (VP1) of about 58 to 60 kDa and a minor capsid protein (VP2). Transmission occurs predominantly through ingestion of contaminated water, food and airborne transmission, as well as contact with contaminated surfaces. The ease with which norovirus is transmitted and the low infectious dose required to establish an infection results in extensive outbreaks in numerous environments, such as hospitals, hotels and schools. There is no antiviral drug available to treat this infection and little is known about its pathogenicity. However, it has been observed that the virus can be taken up by enterocytes where translation of viral nonstructural proteins can occur; it damages and alters intestinal microvilli, leaving them blunt and broadened, thus inhibiting absorption; it causes crypt cell hyperplasia and also leads to apoptosis of enterocyctes. An incubation period of 24-48 hours is usual. Infection is characterized by the acute onset of nausea, vomiting, abdominal cramps, aching limbs, raised temperature and diarrhoea that generally last for about 48 hours. However, more severe and prolonged infection may be observed in children and the elderly. There are five recognized norovirus genogroups, of which three (GI, GII, and GIV) are known to affect humans and, since 2002, variants of the GII.4 genotype have been the most common cause of norovirus outbreaks. There have been 31 different genotypes identified within the genogroups, with a wide degree of genetic variability present even within each genotype.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings

Description 

AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis. 

Features

• Sharp bands
• Suitable for polyacrylamide gel electrophoresis
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 2,000 bp 

Concentration 

45.5 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months 

AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis.