Propargyl-PEG4-bromide is a crosslinker that can be used in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage with azides. The bromide (Br) acts as a good leaving group for nucleophilic substitution reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG4-bromide is a crosslinker that can be used in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage with azides. The bromide (Br) acts as a good leaving group for nucleophilic substitution reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Biotin-PEG8-alkyne
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Biotin-PEG8-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azide groups on a target molecule. The inclusion of a PEG linker in this molecule improves its solubility in water.
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Biotin-PEG8-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azide groups on a target molecule. The inclusion of a PEG linker in this molecule improves its solubility in water.
High Sensitivity RNA Amplification With Improved Detection Isothermal Kit
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Product Description
High Sensitivity RNA Amplification With Improved Detection Isothermal Kit
Product Detail
Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex;Source search and combine the target homology domain, at this time,a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit EXO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(fluorescent type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Fluorescent Probe:Require the suitable length is 46-52nt.
DNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
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It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.
Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
Our Gas PermASeal Heat Seal is made from a non-woven material and is designed for use in cell culture, due to its porous nature
The seal is compatible with polypropylene and polystyrene
It can be removed by peeling, or it can be pierced with a pipette tip manually, using a liquid handling robot.
Gas PermASeal Heat Seal can be utilized for effective overnight incubations, during which it demonstrates significant reductions in evaporation compared to lids
It can also be used for insect and seed storage as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
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Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.