Introduction

The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from Paxgene RNA Tubes
Applications	RT-PCR, qPCR, Northern hybridization, NGS, nucleic acid protection, in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	Blood in the preservation tube
Sample amount	2.5ml
Elution volume	≥30μl
Liquid carrying volume per column	800µl
Binding yield of column	100μg

Principle

The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanolis added to adjust binding conditions, and the lysate is applied to a column.RNA is selectively bound to the silica membrane as contaminants pass through. Remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.

Advantages

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – isolation of several samples can be completed in 40 minutes by using column purification method
• Safety – no phenol chloroform extraction required
• Sensitive – direct lysis of blood, plasma and other samples without separation of leukocytes

Kit Contents

Contents	R416802	R416803
Purification Times	10 Preps	50 Preps
HiPure RNA Mini Columns I	10	50
gDNA Filter Mini Columns	10	50
2ml Collection Tubes	30	150
RNase Free Water	60 ml	250 ml
Buffer MBR1	10 ml	30 ml
Buffer MBR2	5 ml	15 ml
Buffer RW1	15 ml	60 ml
Buffer RW2*	6 ml	20 ml
Proteinase K	12 mg	50 mg
Protease Dissolve Buffer	1.8 ml	5 ml
DNase I	120 µl	600 µl
DNase Buffer	6 ml	30 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Experiment Data

The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Overview

• Isolate high quality DNA from a broad variety of phage strains
• High yields of total DNA
• Fast and easy processing using a rapid spin-column format
• No phenol or chloroform extractions or cesium chloride banding required
• High yields of DNA recovered 3-15 µg DNA from 10⁶-10¹⁰ pfu/ mL of enriched phages

This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.

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Details

Supporting Data

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Kit Specifications
Column Binding Capacity	50 µg
Maximum Column Loading Volume	650 µL
Size of DNA Purified	All sizes
Maximum Amount of Starting Material	1 x 1010 pfu/mL enriched phages
Average Yield*	3-15 µg DNA from 106-1010 pfu/mL of enriched phages
Time to Complete 10 Purifications	45 minutes

* Average yields will vary depending upon a number conditions used and developmental stage.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 46800 (50 preps)	Cat. 46850 (100 preps)
Lysis Buffer B	40 mL	2 x 40 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Buffer B	8 mL	2 x 8 mL
Spin Columns	50	100
Collection Tubes	50	100
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1

TG16E Technical Parameter：

Max. Speed	16000rpm
Max. RCF	19040×g
Max. Capacity	6×10ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	370×290×215mm
Net Weight(Kg)	16KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for TG16E

Order No	Rotor	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
16E-1	Angle rotor	14000	4×8PCR	12070
16E-2	Angle Rotor	16000	12×1.5/2ml	17940
16E-3	Angle Rotor	14000	24×1.5/2ml	17950
16E-4	Angle Rotor	16000	10×5ml	17880
16E-5	Angle Rotor	13000	6×10ml	14190
16E-6	Angle Rotor	12000	24 pieces capillary vessel	15800
16E-7	Angle Rotor	16000	40×0.2ml	19040
16E-8	Angle Rotor	13000	40×0.5ml	17210
16E-9	Angle Rotor	13000	30×0.5ml	13900

Features:
1. Brushless motor, no pollution, free-maintenance.
2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting, operate simply.
3. Electric lid lock, super speed, imbalance protection. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable.
4. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
5. 3 tiers protection steel cover and get the ideal centrifugation result