Propargyl-PEG4-(CH2)3-methyl ester consists of a propargyl group and a methyl ester. The propargyl group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions to form a stable triazole linkage. Under strong basic condition, methyl ester can be hydrolyzed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG4-(CH2)3-methyl ester consists of a propargyl group and a methyl ester. The propargyl group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions to form a stable triazole linkage. Under strong basic condition, methyl ester can be hydrolyzed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Propargyl-PEG14-acid is an alkyne reagent with a carboxylic acid. The carboxylic acid can form amide bonds with amines under the activation of EDC or HATU. The alkyne group can react with azides through Click Chemistry reaction to form triazole linkage. The PEG spacer increases the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG14-acid is an alkyne reagent with a carboxylic acid. The carboxylic acid can form amide bonds with amines under the activation of EDC or HATU. The alkyne group can react with azides through Click Chemistry reaction to form triazole linkage. The PEG spacer increases the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus uberis is a gram-positive bacterium that is known worldwide as an environmental pathogen responsible for a high proportion of cases of mastitis in lactating cows and is also the predominant organism isolated from mammary glands during the non-lactating period. Often it is resistant to treatment and causes persistent high somatic cell counts without clinical mastitis.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.