Propargyl-PEG4-(CH2)3-methyl ester

N-(Acid-PEG2)-N-bis(PEG2-propargyl) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Acid-PEG2)-N-bis(PEG2-propargyl) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Sample collection is non-invasive and painless
• Fast and easy processing using a magnetic bead system
• Isolate high quality genomic DNA
• This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices
• Also available in a 96-well format that can be integrated with a robotic automation system

Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva.  Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	2-5 μg
Average Purity (OD260/280)	1.6 – 1.8
Time to Complete 10 Purifications	30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. RU55400 (50 preps)	Cat. RU62900 (192 preps)
Lysis Buffer F	30 mL	2 x 30 mL
Proteinase K in Storage Buffer	1.2 mL	4 mL
Magnetic Bead Suspension	2 x 1.1 mL	8.5 mL
Elution Buffer B	8 mL	30 mL
Elution Tubes (1.7 mL)	50	–
96-Well Plate	–	2
96-Well Elution Plate	–	2
Adhesive Tape	–	2
Product Insert	1	1

N-(Propargyl-PEG4)-biocytin is a biotin PEG reagent that is reactive with azide containing molecule via click chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Propargyl-PEG4)-biocytin is a biotin PEG reagent that is reactive with azide containing molecule via click chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.