Propargyl-PEG4-(CH2)3-methyl ester

K-PullG6

SKU: 700004329

100/200 assays per kit

PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-6³-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

Explore more of our assay kit products for enzyme activity measurement.

Advantages

• High sensitivity 
• Suitable for manual and auto-analyser formats 
• No transglycosylation interference 
• Very cost effective 
• All reagents stable for > 1 year after preparation 
• Very specific
• Simple format
• Standard included

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.

RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.

– half-life at 95°C of >40 min.

– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (<57°C)

– facilitates “zero-step” RT-PCRs directly from RNA templates (without an isothermal reverse transcription step), as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step.

– allows reverse transcription reactions at high temperatures, thus minimizing the problems encountered with strong secondary structures in RNA that melt at elevated temperatures.

– RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes

Please note: Due to the thermostable nature of the enzyme, it is recommended to design very high melting primers and probes (>65°C). See more details in FAQ.

For research use and further manufacturing.

In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.

RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.

– half-life at 95°C of >40 min.