Propargyl-PEG4-CH2CO2tBu

Overview

• Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
• Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
• Can process challenging samples such as mastitic milk
• Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
• Fast and efficient spin-column format

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example.  The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​

Details

Supporting Data

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Kit Specifications
Maximum Milk Input	1 mL
Time to Complete 10 Purifications	1 hour
DNA Yield*	500 ng to 8 μg
Bacteria Species Processed	Gram positive and Gram negative
Minimum Detection Limit	10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Component	Cat. 21550 (50 preps)
Resuspension Solution A	6 mL
Buffer SK	60 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
Lysozyme (powder)	120 mg
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Propargyl-PEG9-bromide consists of a propargyl group and a bromide group. The propargyl group reacts with azide compounds in copper catalyzed Click Chemistry reactions. The bromide (Br)can be used as a leaving group for nucleophilic substitution reactions. The 9-unit PEG spacer improves the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG9-bromide consists of a propargyl group and a bromide group. The propargyl group reacts with azide compounds in copper catalyzed Click Chemistry reactions. The bromide (Br)can be used as a leaving group for nucleophilic substitution reactions. The 9-unit PEG spacer improves the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Free of all DNases, RNases and nucleic acids
• Prepared without the use of DEPC
• Room temperature storage

Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR.  Our water is prepared without the use of DEPC, and is ready to use without further treatment.

Available Sizes

• 4 x 1.25 mL
• 100 x 1.25 mL
• 100 mL
• 500 mL
• 1000 mL

Details

Applications
For use in any molecular biology application

Storage Conditions
This kit is stable for 2 years after the date of shipment.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.