Propargyl-PEG4-Ms has an alkyne group and a mesylate group. The mesylate group is a sulfonate ester for nucleophilic substitution reactions (SN2). The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environments. Reagent grade, for research use only.
Detail
Propargyl-PEG4-Ms has an alkyne group and a mesylate group. The mesylate group is a sulfonate ester for nucleophilic substitution reactions (SN2). The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environments. Reagent grade, for research use only.
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Bioprocessing with Salt Active Nucleases – Physiological Conditions
M-SAN HQ – Peak performance where it matters most at physiological conditions
Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations between 125 – 250 mM. Due to its excellent performance at physiological conditions, M-SAN HQ can be used directly in the cell medium or the harvested supernatant without buffer adjustments. This makes M-SAN HQ ideal for the manufacturing of fragile vectors such as lentiviruses and retroviruses.
M-SAN HQ can be directly used in medium without buffer adjustments The high activity of M-SAN HQ at standard cell medium conditions leads to improved DNA clearance compared to other commonly used nucleases. In the data (see Figure 6), an over 2-fold reduction in residual DNA was achieved.
Key Benefits
Compatibility: Ideal for working with both fragile and robust viral vectors and proteins in a variety of cell media
Optimum activity: Optimization for cell media salinity allows both shorter DNA fragments and reduced incubation times.
Cost-Effectiveness: Reduced need for additional reagents and steps can lower production costs
Quality: Maintains the integrity of sensitive or labile biological molecules, ensuring a higher quality end product
Flexibility: Can be used directly in a variety of media without the need for customization
Key Features
High purity (≥ 99%)
No protease detected
Endotoxin-tested
Animal origin-free production
Supplied with extended product documentation
Adapted to use in medium without salinity adjustments
Optimal activity at physiological salinity and pH makes it ideal for DNA removal from mammalian cell media. (see Figures)
The high activity of M-SAN HQ at the physiological salinity and pH found in standard cell medium conditions leads to improved DNA clearance compared to commonly used nucleases.
Simple to Optimize in Cell Media
M-SAN HQ is uniquely formulated to excel in physiological pH conditions, offering high performance at the commonly used cell media pH of 7.4.
While other nucleases often require more alkaline environments, M-SAN HQ stands out for its adaptability and effectiveness in cell media. It’s the nuclease that truly aligns with your bioprocessing needs. (See Figure section)
M-SAN HQ ELISA Kit
The M-SAN HQ ELISA Kit confirms the removal of M-SAN High Quality in bioprocessing and biomanufacturing applications with high accuracy:
Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.
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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.
Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.
NGS library size selection with dual clean-ups.
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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.
NGS library size selection with single clean-up for >5 kb and >10 kb libraries.
DBCO-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The highly reactive DBCO group can be used in copper-free click reactions. The TFP ester can react with primary amine groups and is also less susceptible to undergo hydrolysis compared to NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The highly reactive DBCO group can be used in copper-free click reactions. The TFP ester can react with primary amine groups and is also less susceptible to undergo hydrolysis compared to NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.